广西产兰香草HPLC指纹图谱分析  

HPLC Fingerprint Analysis of Caryopteris incana (Thunb.) Miq.from Guangxi

作　　者：甄丹丹[1] 何柳艳[2] 莫缓恒 唐春丽[3] 张淼[1] 甄汉深[1] ZHEN Dandan;HE Liuyan;MO Huanheng;TANG Chunli;ZHANG Miao;ZHEN Hanshen(Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi,530001,China;Guangxi Health College,Nanning,Guangxi,530023,China;The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi,530003,China)

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西卫生职业技术学院,广西南宁530023 [3]广西中医药大学第一附属医院,广西南宁530003

出　　处：《广西科学》2019年第5期522-526,共5页Guangxi Sciences

基　　金：广西一流学科建设项目重点课题（2018XK052）;广西壮族自治区卫生厅中医药科技专项（GZBZ14-07）;壮瑶药协同创新中心项目（桂教科研[2013]20号）;广西壮瑶药重点实验室项目（桂科基字[2014]32号）;广西重点学科壮药学项目（桂教科研[2013]16号）;广西八桂学者中药创新理论与药效研究项目（J13162）资助

摘　　要：以兰香草为研究对象,建立兰香草高效液相色谱(HPLC)指纹图谱分析方法,为今后的质量标准研究提供科学依据。采用高效液相色谱法对不同产地的兰香草进行指纹图谱分析,建立兰香草的HPLC指纹图谱。检测波长为300nm,梯度洗脱,在100min内记录药材的指纹图谱。HPLC指纹图谱标定了8个共有峰。10批兰香草指纹图谱相似度较好,各批药材与对照指纹图谱间的相似度均在0.9以上。HPLC指纹图谱的建立,初步为兰香草的质量标准的制定提供参考。Taking Caryopteris incana(Thunb)Miq.as the research object,the HPLC fingerprint analysis method was established to provide scientific basis for future quality standard research.Fingerprint analysis of Caryopteris incana(Thunb)Miq.from different habitats was carried out by high performance liquid chromatography,and the HPLC fingerprint of Caryopteris incana(Thunb)Miq.was established.The detection wave length was 300 nm,after gradient elution,the fingerprint of the medicinal material was recorded within 100 min.HPLC fingerprints were calibrated for 8 common peaks.The similarity of the fingerprints of 10 batches of Caryopteris incana(Thunb)Miq.was better,and the similarity between the batches of herbs and the control fingerprints was above 0.9.The establishment of HPLC fingerprints provides a preliminary reference for the development of quality standards for Caryopteris incana(Thunb)Miq.

关 键 词：兰香草 高效液相色谱 指纹图谱 成分分析 中药 

分 类 号：R284.1[医药卫生—中药学]