山药块茎膨大期淀粉合成关键酶活性及调控基因的表达分析  

Analysis of key enzyme activity and gene expression in starch synthesis during tuber expansion of yam

作　　者：赵令敏 邵盈[1] 张艳芳[1] 敖兰吉亚 季祥 苏彩霞[2] 霍秀文[1] ZHAO Lingmin;SHAO Ying;ZHANG Yanfang;AOLAN Jiya;JI Xiang;SU Caixia;HUO Xiuwen(College of Horticulture and Plant Protection;Inner Mongolia Agricultural University(Hohhot 010019,China;Department of Agriculture,Animal Husbandry and Bioengineering,Xing An Vocational and Technical College,Wulanhhot 137400,China)

机构地区：[1]内蒙古农业大学园艺与植保学院,内蒙古呼和浩特010011 [2]兴安职业技术学院农牧与生物工程系,内蒙古乌兰浩特137400

出　　处：《河南农业大学学报》2019年第6期847-854,共8页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(31860558);内蒙古农业大学科技创新(培育)团队项目(NDPYTD2013-3)

摘　　要：为了探究山药块茎膨大期淀粉合成、积累与淀粉合成关键酶及其相关调控基因表达的关系,以淀粉含量差异显著的河北安平白山药(Dioscorea opposita Thunb.)和毕克齐长山药(Dioscorea opposita Thunb.)为试验材料,测定块茎膨大期块茎中淀粉、支链淀粉、直链淀粉含量及比例(支链淀粉/直链淀粉)和淀粉合成关键酶(AMY、ADPGase、SPS、SSS)活性,并采用实时荧光定量PCR对块茎中淀粉合成关键酶基因(SPS 1、Isoamylase 3、PFK 3、Starch synthase 3)表达进行分析。结果表明,AMY、ADPGase及SSS对淀粉的合成起关键作用,ADPGase可能是直链淀粉合成的关键酶,SPS和AMY可能对支链淀粉合成起作用。PFK 3基因在山药淀粉合成过程中起重要作用,且PFK 3基因和Starch synthase 3基因可能对直链淀粉的合成起重要作用。In order to investigate the relationship between starch synthesis and accumulation and the expression of key enzymes and related regulatory genes in tuber expansion of yam,Hebei Anpin Bai yam(Dioscorea opposita Thunb.)and Bikeqi Chang yam(Dioscorea opposita Thunb.)with significant differences in starch content were used as test materials to determine the content and proportion of starch,amylopectin and amylose in tuber during tuber expansion(amylopectin/amylose)and the activity of key enzymes(AMY,ADPGase,SPS,SSS)in starch synthesis.The expression of key enzyme genes(SPS1,Isoamylase3,PFK3,Starch synthase3)of starch synthesis in tubers was analyzed by real-time fluorescence quantitative PCR.The results showed that AMY,ADPGase and SSS played a key role in starch synthesis,suggesting that ADPGase might be the key enzyme of amylose synthesis,and SPS and AMY might play an important role in amylopectin synthesis.PFK3 gene played an important role in starch synthesis of yam,and PFK3 gene and Starch synthase3 gene might play an important role in the synthesis of amylose.

关 键 词：山药 块茎膨大期 淀粉合成 酶活性 基因表达 

分 类 号：S632.1[农业科学—蔬菜学]