干旱胁迫对豫杂一号泡桐基因表达谱的影响  

Effect of drought stress on gene expression profile of Paulownia tomentosa×Paulownia fortunei

作　　者：谢博洋 曹喜兵[1] 张雯宇 范国强[1] XIE Boyang;CAO Xibing;ZHANG Wenyu;FAN Guoqiang(Institute of Paulownia,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学泡桐研究所

出　　处：《河南农业大学学报》2019年第6期876-883,共8页Journal of Henan Agricultural University

基　　金：国家重点研发计划项目(2017YFD060050604)

摘　　要：为深入了解豫杂一号泡桐响应干旱胁迫的分子机制,以豫杂一号泡桐为材料,利用RNA-Seq测序技术对干旱胁迫后的豫杂一号泡桐的基因表达变化进行分析。通过比对分析,共鉴定出21911个差异表达基因,对这些差异基因进行GO功能分类,结果显示差异基因共参与了53个类别,集中在细胞、细胞过程、催化等功能类别。KEGG代谢通路分析结果显示,差异表达基因主要参与了“代谢通路”“次生代谢物生物合成”“光合作用”和“苯丙基生物合成”。其中,水通道蛋白、糖转运蛋白、热休克蛋白70和胚胎发育晚期丰富蛋白差异表达量显著,这些基因可能是潜在的干旱胁迫响应基因。采用qRT-PCR技术验证了随机挑选的8个差异表达基因,结果与转录组测序数据趋势一致。In order to understand the molecular mechanism of Paulownia tomentosa×Paulownia fortunei(P.Yuza 1)in response to drought stress,the P.Yuza 1 was used as experimental material,and RNA-Seq technology was used to research changes of genes in response to drought stress.Comparison analy-sis was performed,and a total of 21911 differentially expressed genes related to drought stress were i-dentified in two libraries.GO function classification showed that the differentially expressed genes were involved in 53 categories,and most of them involved in cells,cellular process,catalysis and other classi-fications.KEGG pathway analysis indicated that these differentially expressed genes were mainly in-volved in the synthesis of metabolic pathways,biosynthesis of secondary metabolites,photosynthesis and phenylpropanoid biosynthesis.Among them,the expression levels of aquaporin,SUT,HSP70,and late embryogenesis abundant protein were significantly different,suggesting that these genes may be poten-tial genes of drought response.In addition,qRT-PCR technology was used to validate the expression da-ta measured by RNA-seq for 8 randomly selected differentially genes,and the results were consistent with the trend of transcriptome sequencing data.

关 键 词：豫杂一号泡桐 干旱胁迫 差异表达基因 转录组 

分 类 号：S792.43[农业科学—林木遗传育种]