湿热泄泻模型大鼠的尿液代谢组学研究  

Urine metabolomics in the rat model of damp-heat diarrhea

作　　者：彭晓婷 马琪 张晓松[1] 华永丽[1] 纪鹏[1] 姚万玲 魏彦明[1] PENG Xiaoting;MA Qi;ZHANG Xiaosong;HUA Yongli;JI Peng;YAO Wanling;WEI Yanming(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Institute of Animal Science,Southwest University,Chongqing 402460)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]西南大学动物科学学院,重庆402460

出　　处：《中国实验动物学报》2019年第6期700-708,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(31560709,31860717);国家肉牛/牦牛产业技术体系(CARS-37)~~

摘　　要：目的应用基于超高效液相色谱-串联四级杆飞行时间二级质谱联用(UPLC-Q/TOF-MS/MS)尿液代谢组学方法筛选湿热泄泻潜在代谢标志物,探讨大鼠湿热泄泻发病机制。方法采取高糖高脂(HF)+高温高湿(HH)+腹腔注射产肠毒性大肠杆菌(EC)建立大鼠湿热泄泻模型,采集大鼠造模各阶段(高糖高脂,高温高湿,攻毒)尿液样本,应用尿液代谢组学技术分析样本,多元统计分析方法分析代谢数据。结果筛选出左旋谷酰基-左旋半胱氨酸、左旋甲酰犬尿氨酸、5-羟基-N-甲酰犬尿氨酸、核黄素-5-磷酸、亚油酸、左旋色氨酸和褪黑素等7个潜在代谢标志物。结论湿热泄泻涉及色氨酸代谢、核黄素代谢与亚油酸代谢等代谢通路,表明大鼠体内出现能量、氨基酸及脂类代谢紊乱,本研究结果为揭示中(兽)医湿热泄发病机制提供了参考依据。Objective The ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass/mass spectrometry(UPLC-Q/TOF-MS/MS)urine metabolomics method was used to screen for potential metabolic markers of rat model of damp-heat diarrhea and to explore the pathogenesis.Methods The rat model of damp-heat diarrhea was established using a high-sugar and high-fat diet(HF),a high-temperature and high-humidity environment(HH),and intraperitoneal injection of Escherichia coli(EC).Urine samples were collected from rats at different stages of modeling(HF,HH,and EC)and analyzed using UPLC-Q/TOF-MS/MS.The data were analyzed by multivariate statistical analysis.Results Seven potential endogenous biomarkers were screened,namely,L-glutamic acid-L-cysteine,L-formyl kynurenine,5-hydroxy-N-formylkynurenine,riboflavin-5-phosphate,linoleic acid,L-tryptophan and melatonin.Conclusions The metabolic pathways involved in damp heat diarrhea include metabolic pathways such as tryptophan metabolism,riboflavin metabolism,and linoleic acid metabolism.The results suggest that energy-,amino acid-and lipid-metabolism disorders occur in rats with damp-heat diarrhea which provide a scientific basis for elucidating the mechanism of pathogenesis of Chinese medicine and traditional Chinese veterinary medicine damp-heat diarrhea.

关 键 词：湿热泄泻 超高效液相色谱-串联四级杆飞行时间二级质谱联用 尿液 代谢组学 生物标志物 大鼠 

分 类 号：Q95-33[生物学—动物学]