青海湖裸鲤胚胎不同发育时期TRH mRNA的原位杂交  

In situ hybridization of TRH mRNA at different periods in the development of Gymnocypris przewalskii

作　　者：梁志康 冯静雯 谢保胜 LIANG Zhikang;FENG Jingwen;XIE Baosheng(Kunlun College,Qinghai University,Xi’ning 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,College of Ecol-Environmental Engineering,Qinghai University,Xi’ning 810016)

机构地区：[1]青海大学昆仑学院,西宁810016 [2]高原生态与农业国家重点实验室,青海大学生态环境工程学院,西宁810016

出　　处：《中国实验动物学报》2019年第6期760-764,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(30360632)~~

摘　　要：目的本试验旨在观察和分析青海湖裸鲤的不同发育时期胚胎促甲状腺激素释放激素(thyrotropin-releasing hormone)TRH基因的表达情况。方法提取青海湖裸鲤的总RNA制备地高辛标记的反义TRH基因的mRNA探针,用体视显微镜观察原位杂交的TRH基因表达情况。结果显微观察显示青海湖裸鲤胚胎发育第6~8天在间脑出现了TRH基因的阳性杂交信号;第9~11天的胚胎的间脑、中脑、后脑和髓脑多处出现阳性杂交信号,间脑的表达量比其他脑组织的表达量高。结论原位杂交表明THR基因在青海湖裸鲤6~11 d胚胎中表达,提示促甲状腺激素释放激素可能对青海湖裸鲤脑部的发育和成熟有重要的作用。Objective This experiment is aimed to analyze the expression state of the thyrotropin releasing hormone(TRH) gene at different periods in the development of Gymnocypris przewalskii("Przewalskii′s naked carp") embryos. Methods The total RNA of G. przewalskii was extracted to make digoxin-labelled antisense mRNA of the TRH gene. In situ hybridization technology and stereomicroscopy were used to observe the expression status of the TRH gene in the G. przewalskii embryos. Results Microscopy showed that positive hybridization signals of the TRH gene appeared in the diencephalon in the 6-8-day embryo, and appeared in the diencephalon, midbrain, hindbrain and medulla in the 9-11-day embryo. The expression level in the diencephalon was higher than that in other brain areas. Conclusions In situ hybridization shows that the THR gene is expressed in 6-11-day embryos of G. przewalskii, suggesting that THR may play an important role in the brain development and maturation in G. przewalskii.

关 键 词：青海湖裸鲤 胚胎发育期 原位杂交 促甲状腺激素释放激素 

分 类 号：Q95-33[生物学—动物学]