小麦抗麦红吸浆虫基因标记的开发与验证  

Development and validation of markers linked to genes resistant to Sitodiplosis mosellana in wheat

作　　者：郝志明 耿妙苗 温树敏[1] 闫桂军 王睿辉[1] 刘桂茹[1] HAO Zhi-Ming;GENG Miao-Miao;WEN Shu-Min;YAN Gui-Jun;WANG Rui-Hui;LIU Gui-Ru(Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Baoding 071000,Hebei,China;School of Agriculture and Environment,the University of Western Australia,Nedlands 6009,Australia)

机构地区：[1]河北农业大学/华北作物种质资源研究与利用教育部重点实验室,河北保定071000 [2]西澳大学农业与环境学院,澳大利亚尼德兰兹6009

出　　处：《作物学报》2020年第2期179-193,共15页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31371617)资助~~

摘　　要：麦红吸浆虫(Sitodiplosis mosellana Géhin)严重影响小麦产量和品质,选育和使用抗虫品种是降低虫害损失最安全有效的途径,利用抗虫性连锁或功能标记对提高小麦抗虫分子育种效率具有重要意义。在前期从转录组数据中挖掘到抗虫性主效QTL(QSm.hbau-4A)6个相关差异基因的基础上,依据这些基因序列中存在的InDel和SNP,分别开发了2个EST标记和6个KASP标记,并在抗虫性不同的一套重组近交系(RIL)和一套小麦品种中进行了标记的有效性验证。所开发的8个标记在抗、感虫小麦亲本间均表现出较好的多态性,在RIL株系中的检测有效率均达到90%左右;除E10-10外,这些标记在供试高抗(56.3%~86.7%)和高感(85.7%~100.0%)小麦品种中的检测有效率均较高,可用于小麦种质资源的抗虫性筛选。同时发现,11个具备所有抗虫标记位点的抗虫小麦品种,多为审定时间较早或已停止使用的品种,这使得结合标记辅助选择等手段鉴定和创新小麦抗虫种质资源的工作日益紧迫。Orange wheat blossom midge(OWBM)(Sitodiplosis mosellana Géhin)has seriously reduced wheat production and processing quality.Breeding midge-resistant wheat has been considered the most effective way to reduce kernel losses caused by OWBM,and marker-assisted selection(MAS)strategy in crop breeding using linked or functional markers of target trait of interest is of great importance in improving breeding efficiency.Based on the InDels and SNPs within the sequences of six midge resistance-related genes discovered from transcriptome data in the previously mapped major QTL(QSm.hbau-4A)region,we developed and validated two EST and six KASP markers in a panel of recombinant inbred lines(RILs)and a panel of wheat cultivars with different OWBM resistance levels.These markers were polymorphic between the resistant and susceptible wheat parents,and approximately 90% of RIL lines showed the corresponding marker-based genotypes with their phenotypes.Except for E10-10,the other seven markers had higher detection efficiency in highly resistant(56.3%−86.7%)and in highly susceptible(85.7%−100.0%)wheat cultivars,thus making them applicable for screening midge-resistant wheat germplasm with locus QSm.hbau-4A.Among the eleven midge-resistant wheat cultivars with all resistance alleles for the seven markers developed,most were historical wheat cultivars,and rarely used in the present production,which suggests how to use old cultivars in wheat germplasm identification and enhancement on midge-resistance through MAS is urgent.

关 键 词：小麦 麦红吸浆虫 抗虫相关基因 功能标记 SNP EST KASP 

分 类 号：S51[农业科学—作物学]