21日龄CD163基因编辑猪血液生理生化及免疫指标的测定和分析  

Determination and Analysis of Blood Physiological, Bio-chemical Parameters and Immunity of 21-day-old CD163 Gene Editing Pigs

作　　者：张立苹[1] 吴常宝 牟玉莲[3] 周伟良[4] 华再东[1] 华文君[1] 李奎[3] 毕延震[1] ZHANG Li-ping;WU Chang-bao;MOU Yu-lian;ZHOU Wei-liang;HUA Zai-dong;HUA Wen-jun;LI Kui;BI Yan-zhen(Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding/Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;College of Life Science,Inner Mongolia University,Hohhot 010000,China;Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Tianjin Ningheyuan Swinebreeding Farm,Tianjin 301504,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,湖北武汉430064 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010000 [3]中国农业科学院北京畜牧兽医研究所,北京100193 [4]天津市宁河原种猪场,天津301504

出　　处：《江西农业学报》2019年第12期77-81,共5页Acta Agriculturae Jiangxi

基　　金：国家科技重大专项(2018ZX0800601B-002);转基因生物新品种培育重大专项(2016ZX08006001-005);湖北省农业科技创新中心项目(2019-620-004-001);湖北省农业科学院青年拔尖人才项目(Q2018020)

摘　　要：探讨了CD163基因编辑对21日龄断奶仔猪前腔静脉血液中部分生理生化、免疫球蛋白及补体蛋白等指标的影响。结果表明:CD163基因编辑后,纯合子的淋巴细胞比率、中间细胞比率、红细胞平均体积均显著低于杂合子和阴性猪(P<0.05);纯合子的粒细胞比率、粒细胞数显著高于杂合子和阴性猪(P<0.05);杂合子的血小板总数显著高于纯合子和阴性猪(P<0.05);纯合子的葡萄糖和胆固醇含量显著高于杂合子和阴性猪(P<0.05);纯合子血液中IgG、IgA、IgE和IgM等4类免疫球蛋白的含量均显著高于杂合子和阴性猪(P<0.05);纯合子血液中补体蛋白3和补体蛋白4与杂合子和阴性猪没有显著性差异(P>0.05)。The effects of CD163 gene editing on some blood physiological,bio-chemical,immunoglobulin and complement proteins of the 21-days-old weaned piglets were investigated.The results showed that the lymphocyte ratio,intermediate cell ratio and mean corpuscular volume of homozygote were significantly lower than that of heterozygote and negative pigs(P<0.05)after the editing of CD163 gene,and the granulocyte ratio and granulocytes of homozygote were significantly higher than that of heterozygote and negative pigs(P<0.05).The total number of platelets of heterozygote was significantly higher than that of homozygote and negative pigs(P<0.05).The glucose and cholesterone contents of homozygote were significantly higher than that of heterozygote and negative pigs(P<0.05).The content of 4 kinds of immunoglobulin,such as IgG,IgA,IgE and IgM in the blood of homozygote was significantly higher than that of heterozygote and negative pigs(P<0.05).there was no significant difference among the complement protein 3 and complement protein 4 in the blood of the homozygote,the heterozygote and the negative pig(P>0.05).

关 键 词：基因编辑 猪 血液 生理生化 免疫球蛋白 补体蛋白 

分 类 号：S828.1[农业科学—畜牧学]