基于叶片解剖结构对灰杨不同发育阶段的抗旱性评价  

Evaluation of Drought Resistance of Different Development Stages of Populus pruinosa Based on the Leaf Anatomical Structure

作　　者：翟军团 韩晓莉 李志军 Zhai Juntuan;Han Xiaoli;Li Zhijun(College of Life Sciences,Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xinjiang Production&Construction Corps.Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学学院/新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室

出　　处：《塔里木大学学报》2019年第4期12-21,共10页Journal of Tarim University

基　　金：国家自然科学基金项目“水分变化对胡杨异形叶性时空变化的调节机制”(31860198);国家自然科学基金新疆联合基金项目“塔里木河流域胡杨雌雄干旱适应差异的生理与分子机制”(U1803231)

摘　　要：基于灰杨异形叶解剖结构,对灰杨9个发育阶段的抗旱性进行分析。通过常规石蜡制片技术,测定灰杨叶片上下表皮、栅栏组织、海绵组织和叶片厚度等10项解剖结构指标参数,运用主成分分析和隶属函数法对灰杨不同发育阶段进行抗旱性分析与评价。结果表明,灰杨异形叶解剖结构随发育阶段而变化,表现为表皮细胞数、栅栏组织细胞数、栅栏组织厚度、叶片厚度、栅/海比均随径阶增加而增加;海绵组织厚度、叶片组织结构疏松度则均随径阶增加而减少,这些指标参数与胸径呈极显著/显著正负相关关系。通过主成分分析法结合各指标的变异系数筛选出5项具有代表性的抗旱评价指标:叶片厚度、栅栏组织厚度、表皮细胞数、栅栏组织细胞数、栅/海比,运用隶属函数值法对灰杨各发育阶段的抗旱性能评分排序,灰杨9个发育阶段的抗旱性由强到弱依次为:18径阶>16径阶>12径阶>14径阶>10径阶>8径阶>6径阶>4径阶>2径阶,灰杨的抗旱性随个体发育阶段逐渐增强。Based on the anatomical structure of heteromorphic leaf,the drought resistance of nine development stages of Populus pruinose was analyzed.Ten indicators of leaf structure,such as epidermis,palisade tissue,spongy tissue and leaf thickness,et al.,were compared by conventional paraffin sectioning technique.The principal component analysis and membership function method were used to evaluate the drought resistance of different development stages of P.pruinosa.Resulst revealed that the anatomical structure of heteromorphic leaves of P.pruinosa changed with the development stages,which showed that the epidermal cells numbers,palisade tissue cell snumbers and palisade tissue thickness,leafthickness,palisadetissu/sponge tissue ratio all increase as the diameter class increases.The thickness of the spongy tissue and the looseness of leaf structure tissue decrease with the increase of the diameter class,these indicators have significant differences between different diameter class.Five representative drought resistance evaluation indexes were selected by principal component analysis combined with the coefficient of variation of each indicators:leafthickness,palisade tissue thickness,epidermal cell number,palisade tissue cell number,palisadetissu/spongy tissue ratio,Ranking of drought resistance performance of development stages of P.pruinosa by membership function method.The drought resistance scores of different development stages of P.pruinosa were ranked as follows:18 diameter>16 diameter class>12 diameter class>14 diameter class>10 diameter class>8 diameter class>6 diameter class>4 diameter class>2 diameter class.In the process of individual development,drought resistance gradually increases with ontogenetic development.

关 键 词：灰杨 不同发育阶段 异形叶 解剖结构 抗旱评价 

分 类 号：Q944[生物学—植物学]