我国部分常用玉米种质资源对镰孢菌病害的抗性评价  

Resistance Evaluation of Some Commonly Used Maize Germplasm Resources to Fusarium Diseases in China

作　　者：渠清 李丽娜 刘俊 王绍新[2] 曹志艳 董金皋 QU Qing;LI LiNa;LIU Jun;WANG ShaoXin;CAO ZhiYan;DONG JinGao(College of Life Sciences,Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Baoding 071001,Hebei;Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041)

机构地区：[1]河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室,河北保定071001 [2]石家庄市农林科学研究院,石家庄050041

出　　处：《中国农业科学》2019年第17期2962-2971,共10页Scientia Agricultura Sinica

基　　金：国家现代农业产业技术体系专项(CARS-02)

摘　　要：【目的】玉米穗腐病、茎腐病和鞘腐病是我国玉米产区普遍发生的三大镰孢菌(Fusarium spp.)病害,近年来有严重混和发生的趋势,三大病害主要致病菌分别为拟轮枝镰孢(F.verticillioides)、禾谷镰孢(F.graminearum)和层出镰孢(F.proliferatum)。种植抗病品种是控制该类病害最经济有效的方法,本研究旨在筛选出单抗或兼抗穗腐病、茎腐病和鞘腐病的玉米种质,为玉米品种的科学选育和合理布局提供参考。【方法】选取我国玉米种质B73、B37、郑58、昌7-2、齐319等16个常用育种自交系,将玉米穗腐病主要病原菌拟轮枝镰孢、茎腐病主要病原菌禾谷镰孢及鞘腐病主要病原菌层出镰孢分别接种于玉米果穗、茎秆及叶鞘,具体方法为待整株长到吐丝期用连动注射器将拟轮枝镰孢孢子悬浮液沿花丝通道注射到健康玉米果穗;将健康玉米地上第一节的茎部用针扎孔后向其中注射禾谷镰孢孢子悬浮液;将层出镰孢孢子悬浮液沿健康玉米植株叶基部接种到地上2—5节间的叶鞘内。以上各种接种方式均使用无菌水作为对照。在2016年和2018年度进行人工田间接种试验,通过评价穗腐病、鞘腐病的病情指数以及茎腐病发病面积,评估所选自交系对以上镰孢菌引起的穗腐病、茎腐病和鞘腐病的抗性级别。【结果】供试自交系中吉853、OH43和X178对穗腐病表现为中抗,B73、B37、PH6WC、掖478、郑58、9058、昌7-2、浚928、Mo17、A619、PH4CV、齐319和13-1077共13份材料为感病或高感;齐319、PH4CV、Mo17、9058、B37、B73、昌7-2和13-1077共8份材料对茎腐病表现为中抗或高抗,郑58、掖478、PH6WC、浚928、吉853、A619和OH43共7份材料表现为感病或高感;B73、B37、郑58、掖478、PH6WC、9058、昌7-2、吉853、浚928、Mo17、A619、PH4CV、OH43、齐319和13-1077共15份材料对鞘腐病表现为中抗或抗病。从16个自交系所在种群来看,瑞德群对穗腐病表现为�【Objective】Maize ear rot,stalk rot and sheath rot are the three common Fusarium diseases in maize producing areas in China,and there is a trend of serious mixing occurrence in recent years.The main pathogens of the three major diseases are F.verticillioides,F.graminearum and F.proliferatum.Planting resistant varieties is the most economical and effective method to control this kind of diseases.The objective of this study is to screen out the maize resistant germplasms to ear rot,stalk rot and sheath rot,and to provide references for scientific breeding and rational layout of maize varieties.【Method】Sixteen common inbred lines of maize including B73,B37,Zheng 58,Chang 7-2,Qi 319 and so on were selected to inoculate F.verticillioides,F.graminearum,and F.proliferatum on maize ears,stalks and sheaths,respectively.The suspension spores of F.verticillioides were injected into healthy maize ears along the filament channel with a continuous syringe at silk stage.The stem of the first aboveground segment of healthy maize was pinned and then injected with the suspension spores of F.graminearum.The suspension spores of F.proliferatum were injected into the sheath of the aboveground 2-5 internodes along the base of healthy maize sheath.Sterile water was used as control.In 2016 and 2018,experiments were conducted in fields to evaluate the disease index of ear rot,sheath rot and the lesion areas of stalk rot,so as to evaluate the resistance level to the ear rot,stalk rot and sheath rot【.Result】The tested inbred lines Ji 853,OH43 and X178 were moderate resistance to ear rot,13 of the tested inbred lines such as B73,B37,PH6WC,Ye 478,Zheng 58,9058,Chang 7-2,Xun 928,Mo17,A619,PH4CV,Qi 319 and 13-1077 were sensitivity or high sensitivity to ear rot;8 of the tested inbred lines including Qi 319,PH4CV,Mo17,9058,B37,B73,Chang 7-2 and 13-1077 were moderate resistance and high resistance to stalk rot,7 of the tested inbred lines such as Zheng 58,Ye 478,PH6WC,Xun 928,Ji 853,A619 and OH43 were sensitivity or high sensitivity t

关 键 词：玉米种质资源 穗腐病 茎腐病 鞘腐病 抗性评价 镰孢菌 

分 类 号：S51[农业科学—作物学]