男性不育患者精液中UU、CT感染及AsAb抗体与免疫性不育的关系  

The Relationship Between UU,CT Infections,AsAb in Semen and Immunological Infertility of Male Patients

作　　者：刘玖 钱莉 刘佳欣 张盈盈 宿炳忠 LIU Jiu;QIAN Li;LIU Jia-xin;ZHANG Ying-ying;SU Bing-zhong(Sichuan Maternal and Child Health Hospital,Chengdu 610041,China;Rehabilitation Center of Sichuan Vocational College of Physical Education,Chengdu 610041,China;Department of Clinical Laboratory,Sichuan Electric Power Hospital,Chengdu 610000,China)

机构地区：[1]四川省妇幼保健院,四川成都610041 [2]四川体育职业学院康复中心,四川成都610041 [3]四川电力医院检验科,四川成都610000

出　　处：《标记免疫分析与临床》2019年第11期1867-1871,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的分析男性不育患者精液中解脲支原体(UU)、沙眼衣原体(CT)感染及抗精子抗体(AsAb)与免疫性不育的关系。方法选取2017年8月至2019年3月我院泌尿生殖男科和生殖医学中心就诊的104例不育男性患者(观察组)、50例已生育健康男性(对照组)为研究对象,采集研究对象的精液标本,采用微生物检验法测定UU感染率,采用乳胶免疫层析法测定CT感染率,并记录AsAb抗体阳性率,应用MAKLER计数板人工精液分析方法检测精液参数,分析UU、CT感染及AsAb阳性率与精液参数的关系。结果观察组UU感染率、CT感染率、AsAb阳性率均高于对照组(P<0.01);AsAb阳性者中,UU、CT感染率均高于AsAb阴性者(P<0.05);观察组精液液化时间较对照组延长,观察组的精子浓度、精子存活率、前向运动较对照组低,而精子畸形率高于对照组(P<0.05),观察组中UU、CT感染及AsAb阳性者精液液化时间延长,而精子浓度、精子存活率、前向运动低于UU、CT感染及AsAb阴性患者,UU、CT感染及AsAb阳性者精子畸形率高于UU、CT感染及AsAb阴性患者(P<0.05)。结论精液中UU、CT感染及AsAb抗体与免疫性不育有密切关系,且对患者精液参数有一定影响,是引起男性不育的重要原因。Objective To analyze relationship between Ureaplasma urealyticum(UU),Chlamydia trachomatis(CT)infections,anti-sperm antibody(AsAb)in semen and immunological infertility of male patients.Methods 104 infertile male patients(the observation group)who were treated in the genitourinary andrology and reproductive center of the hospital from August,2017 to March,2019 and 50 healthy males(the control group)were selected as study subjects.Semen samples were collected from all the subjects.The UU infection rate and the CT infection rate were determined by microbiological test and latex immunochromatography,respectively.The positive rate of AsAb was recorded.The semen parameters were detected by computer-assisted semen analysis system and artificial semen analysis.The relationship between UU,CT infection,positive rate of AsAb and semen parameters was analyzed.Results The infection rates of UU and CT,and positive rate of AsAb in the observation group were higher than those in the control group(P<0.01).The infection rates of UU and CT were higher in AsAb-positive patients than AsAb-negative ones(P<0.05).The semen liquefaction time in the observation group was longer than that in the control group.The sperm concentration,survival rate of sperm and forward activity were lower than those in the control group,while the sperm deformity rate was higher than the control group(P<0.05).In the observation group,semen liquefaction time was prolonged in patients with UU,CT infection and AsAb-positive,while concentration and survival rate of sperm,and forward activity were lower than those in UU,CT infection and AsAb-negative patients.The sperm deformity rate in patients with UU,CT infection and AsAb-positive was higher than that with UU,CT infection and AsAb-negative(P<0.05).Conclusion UU,CT infection and AsAb in semen are closely related to immunological infertility,which are of certain influences on semen parameters,and are important causes leading to male infertility.

关 键 词：男性不育 精液 UU CT AsAb 免疫性不育 

分 类 号：R71[医药卫生—妇产科学]