玉米品种“家佳荣2号”杂交种子纯度的SNP分子鉴定  

Identification of Purity of Hybrid Maize Varieties ‘Jiajia Rong No.2’ by SNP Molecular Marker

作　　者：姚宗泽 李阳 郭宗娟 李军萍 聂贵芳 赵自仙[1] 李云波[5] YAO Zong-ze;LI Yang;GUO Zong-juan;LI Jun-ping;NIE Gui-fang;ZHAO Zi-xian;LI Yun-bo(College of Agriculture and Biotechnology,Yunnan Agricultural University,Yunnan Kunming 650201,China;Hangzhou China Soft Anren Network Communication Co.Ltd.,Zhejiang Hangzhou 310012,China;Yuxi Agricultural Vocational Technical College,Yunnan Yuxi 653100,China;Wenshan Seed Management Station,Yunnan Wenshan 663099,China;Anning Agricultural Technology Promotion Institute,Yunnan Anning 650300,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]杭州中软安人网络通信股份有限公司,浙江杭州310012 [3]玉溪农业职业技术学院,云南玉溪653100 [4]文山市种子管理站,云南文山663099 [5]安宁市农业技术推广所,云南安宁650300

出　　处：《西南农业学报》2019年第11期2486-2491,共6页Southwest China Journal of Agricultural Sciences

基　　金：云南农业大学博士启动基金项目(A2002276)

摘　　要：【目的】验证SNP分子标记中HRM技术鉴定结果的可靠性,为杂交玉米种子纯度鉴定方法的选择提供参考。【方法】以杂交种家佳荣2号及其父母本、近似杂交组合JR02为材料,采用田间小区种植鉴定和SNP分子标记中的HRM技术两种方法对杂交玉米家佳荣2号的同一批种子样品进行纯度鉴定。【结果】田间小区种植纯度鉴定结果为97.8%,SNP分子标记纯度鉴定结果为97.1%。【结论】SNP分子标记中的HRM技术鉴定结果可靠,可作为杂交玉米种子的纯度鉴定方法之一。【Objective】The purpose of this paper is to verify the reliability of HRM technology identification results in SNP molecular markers,and provide reference for the selection of hybrid corn seed purity identification methods.【Method】In this study,hybrid maize‘Jiajia Rong No.2’and its parents and approximate hybrid combinations‘JR02’were used as the materials to identify the purity of hybrid maize‘Jiajia Rong No.2’by using two methods of field planting identification and SNP molecular marker-HRM technology.【Result】The seed purity by field planting identification was 97.8%and by SNP molecular marker-HRM technology was 97.1%.【Conclusion】The results of identify by SNP molecular marker-HRM technology is reliable,so it can be used as one method of hybrid maize purity identification.

关 键 词：杂交玉米 纯度鉴定 SNP分子标记 HRM技术 

分 类 号：S513[农业科学—作物学]