广西猪群新发感染猪捷申病的检测诊断  

Detection and Diagnosis of Porcine Teschen Disease of Newly Infected Pigs in Guangxi

作　　者：秦毅斌[1] 苏乾莲[1] 赵硕 梁家幸[1] 李斌[1] 卢冰霞[1] 周展宏 陈忠伟[1] 何颖[1] 段群棚[1] 周英宁[1] 蒋冬福[1] 赵武[1] QIN Yi-bin;SU Qian-lian;ZHAO Shuo;LIANG Jia-xing;LI Bin;LU Bing-xia;ZHOU Zhan-hong;CHEN Zhong-wei;HE Ying;DUAN Qun-peng;ZHOU Ying-ning;JIANG Dong-fu;ZHAO Wu(Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology,Guangxi Nanning 530001,China;College of Animal Science and Technology,Guangxi University,Guangxi Nanning 530004,China)

机构地区：[1]广西兽医研究所/广西兽医生物技术重点实验室,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530004

出　　处：《西南农业学报》2019年第11期2699-2703,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西科技重大专项项目(桂科AA17204057);广西自然科学基金项目(2017GXNSFBA198092);广西基本科研业务费专项(桂科专项17-2;19-2);广西兽医生物技术重点实验室开放基金课题(16-380-45-B-3)

摘　　要：【目的】建立猪捷申病毒(PTV)反转录-聚合酶链反应(RT-PCR)的检测方法,并应用于广西猪群PTV感染诊断。【方法】通过对GenBank中登录的PTV不同血清型基因序列,在5’端保守区设计一对特异性引物,经退火温度摸索及特异性和敏感性试验,建立PTV的RT-PCR检测方法,并对广西部分猪场腹泻病例进行猪捷申病的感染诊断。【结果】所建立的方法能特异性地扩增PTV,不能扩增猪流行性腹泻病毒、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒、猪瘟病毒、猪传染性胃肠炎病毒、猪A群轮状病毒、猪C群轮状病毒、猪丁型冠状病毒。建立的方法具有较高的敏感性,可检测低至1.16 pg/μl的质粒模板及12.15 pg/μl的核酸模板。应用建立的RT-PCR方法对2018年3月-2019年4月从广西部分猪场采集的235份病猪腹泻粪便进行PTV临床检测,PTV阳性为24份,阳性率为10.21%。【结论】建立的PTV RT-PCR方法简单特异、灵敏度高,可适用于实验室PTV检测诊断。广西部分受检猪场已存在PTV感染,应予以警惕,加强监测与防控。【Objective】The reverse transcription polymerase chain reaction was developed to detect Porcine teschovirus in swine clinical samples in Guangxi.【Method】Based on sequence analysis of different serotypes in GenBank,the RT-PCR primers were designed with conservative regions at the 5’end,and the RT-PCR assay was established after annealing temperature optimization and specificity and sensitivity test,and to diagnosis Porcine teschovirus infection in Guangxi.【Result】This detection method had high specificity and it can amplify Porcine teschovirus,no detection appeared with Porcine epidemic diarrhea virus,Porcine pseudorabies virus,Porcine reproductive and respiratory syndrome virus,Classical swine fever virus,porcine transmissible gastroenteritis virus,Group A porcine rotavirus,Group C porcine rotavirus,porcine deltacoronavirus.The detection was high sensitive and its limit was as lower as 1.16 pg/μl plasmid template and 12.15 pg/μl cDNA template.The established RT-PCR method was used to detect 235 cases of diarrhea feces collected from pig farms in Guangxi from March 2018 to April 2019,and 24 cases were positive for PTV,with a positive rate of 10.21%.【Conclusion】The established RT-PCR was simple,specific and sensitive assay,which can be used in laboratory PTV detection and diagnosis.PTV infection has been found in some pig farms in Guangxi,so we should be vigilant,strengthen monitoring,prevention and control.

关 键 词：猪捷申病毒 诊断 临床检测 

分 类 号：S828[农业科学—畜牧学]