我国地方鸡种TAP2基因SNP筛查及遗传结构分析  

SNP Screening and Genetic Structure Analysis of TAP2 Gene in Local Chicken Breeds in China

作　　者：王莎莎 刘宗正[1] 张宝珣[1] 刘虎传 郝小静[1] 李培培[1] 黄晓 原小雅 常国斌 WANG Sha-sha;LIU Zong-zheng;ZHANG Bao-xun;LIU Hu-chuan;HAO Xiao-jing;LI Pei-pei;HUANG Xiao;YUAN Xiao-ya;CHANG Guo-bin(Qingdao Animal Husbandry and Veterinary Medicine Research Institute,Qingdao 266000,China;Yangzhou University,Yangzhou 225000,China)

机构地区：[1]青岛市畜牧兽医研究所,山东青岛266000 [2]扬州大学,江苏扬州225000

出　　处：《畜牧与饲料科学》2019年第12期15-19,共5页Animal Husbandry and Feed Science

摘　　要：抗原处理相关转运体基因(TAP)位于主要组织相容性复合体区域,而由TAP1和TAP2组成的二聚体能够将抗原肽从细胞质转运入内质网腔,因此,其在免疫反应过程中发挥重要的呈递作用。[目的]检测26个鸡种TAP2基因全长的序列信息,分析TAP2基因单核苷酸多态性,并进一步研究TAP2不同SNPs位点对其蛋白结构的影响及其在不同鸡种中的遗传变异特点。[方法]采用目标捕获序列测序方法,检测26个鸡种的260个体的TAP2基因全长,利用DNAMAN软件对26个鸡品种的测序序列进行比对分析,筛选不同鸡种TAP2基因存在的SNP位置以及类型,并通过软件预测分析SNP对蛋白的影响,进一步构建系统进化树,探究不同鸡种同源性。[结果]TAP2基因总共有效测序长度为3037 bp,在不同鸡种中一共发生了20个位点的突变,具有较为丰富的遗传多样性,其中9个SNP发生在外显子上,其余则存在于内含子,同时分析了对编码氨基酸产生影响的4个SNP(rs316795220、rs738706078、rs739725385、rs732937653)对蛋白结构的影响。[结论]鸡TAP2基因具有较高的多态性,这些丰富多态的基因的存在为进一步利用丰富的资源提供了原始材料。该研究为TAP2基因转运抗体功能关联研究候选SNPs的选择提供了科学依据。Transporter associated with antigen processing(TAP)genes are located in the major histocompatibility complex(MHC)region,and the dimers composed of TAP1 and TAP2 can transport the antigen peptide from cytoplasm into the endoplasmic reticulum cavity.Therefore,they play an important presentative role in an immune response.[Objective]To further reveal the effect of varied SNPs in TAP2 genes on their proteins structure,and to characterize their genetic variation in different chicken breeds.[Method]Target capture sequencing assay was used to determine the full length of TAP2 genes in 260 chicken individuals of 26 breeds.The multiple sequence alignment analysis among the obtained sequences of 26 chicken breeds was carried out using DNAMAN software to screen the locations and to determine the types of the SNPs in TAP2 genes in 26 chicken breeds.The impact of the SNPs in TAP2 genes on their corresponding proteins structure was predicted via bioinformatics software.The phylogenetic tree was established to assess the homology of TAP2 genes among different chicken breeds.[Result]The effective sequencing length of TAP2 gene was 3037 bp.A total of 20 SNPs were observed in the 26 different chicken breeds,indicating a rich genetic diversity.Among them,9 were found in exons and the remaining ones were located in introns.The effect of four SNPs(rs316795220,rs738706078,rs739725385 and rs732937653)on structure of the proteins encoded by them was characterized.[Conclusion]The TAP2 gene in chicken has rich genetic diversity,providing abundant original materials for further application of these germplasm resources of chickens.Our results give new insights into the studies on selection of candidate SNPs in TAP2 gene involved in antibody transportation function.

关 键 词：鸡 TAP2 SNP 抗性 

分 类 号：S831.892[农业科学—畜牧学]