外源磷对砷胁迫下挺水植物抗氧化酶系统的影响  

Effects of exogenous phosphorus on antioxidant enzyme system of emergent plants under arsenic stress

作　　者：陈天 刘云根[1,2] 王妍[1] 杨桂英[1] 任伟[1] 包宁颖 杜崇宣 CHEN Tian;LIU Yun-gen;WANG Yan;YANG Gui-ying;REN Wei;BAO Ning-ying;DU Chong-xuan(College of Ecology and Soil and Water Conservation,Southwest Forestry University,Kunming 650224,China;Center of Water Science and Engineering,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学生态与环境学院,云南昆明650224 [2]西南林业大学水科学与工程中心,云南昆明650224

出　　处：《江苏农业学报》2019年第5期1040-1046,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(21767027、31760245);云南省应用基础研究计划项目(2018FB044)

摘　　要：为探讨外源磷输入对底泥砷胁迫下挺水植物香蒲(Typha angustifolia L.)抗氧化酶系统的影响,测量0 mg/kg、150 mg/kg、600 mg/kg 3种砷水平胁迫下,输入0 mg/L、2 mg/L、20 mg/L 3种外源磷水平后香蒲抗氧化酶活性变化。结果表明,(1)低质量浓度磷(2 mg/L)和低含量砷(150 mg/kg)处理提高了香蒲鲜质量;(2)外源磷输入刺激了底泥无砷处理下香蒲超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性上升,抑制了谷胱甘肽(GSH)活性,高质量浓度磷(20 mg/L)处理下抑制作用增大,同时也促进丙二醛(MDA)的产生;无外源磷输入时,底泥添加低含量砷处理促进SOD活性和MDA积累量上升,高含量砷(600 mg/kg)处理抑制了CAT和GSH活性;(3)低质量浓度外源磷输入提高了底泥砷胁迫处理下香蒲体内抗氧化酶活性,降低MDA积累量。外源磷提高了底泥砷胁迫下香蒲抗氧化酶系统活性,但是这种刺激作用有一定的限度。香蒲比一般蕨类和苔藓类植物更有生长优势,所以香蒲具有修复面临的磷富营养化和砷污染双风险湿地的巨大潜力。To investigate the effects of exogenous phosphorus(P)input on the antioxidant enzyme system of Typha angustifolia L.under arsenic(As)stress,the antioxidant enzyme activities of typha were measured at 0 mg/kg,150 mg/kg and 600 mg/kg As levels after 0 mg/L,2 mg/L and 20 mg/L of exogenous P input.The results showed that the fresh weight of typha was increased under the treatment of low mass concentration P(2 mg/L)and low content As(150 mg/kg).Exogenous P input stimulated the increase of superoxide dismutase(SOD)and catalase(CAT)activities,inhibited glutathione(GSH)activity,and accelerated the inhibition of high mass concentration P(20 mg/L)and malondialdehyde(MDA)production in As-free sediment habitats.In the absence of exogenous P input,low content As stimulated the increase of SOD activity and MDA content in typha leaves,and high content As(600 mg/kg)inhibited the activities of CAT and GSH.Treatment of low mass concentration exogenous P is beneficial to the improvement of antioxidant enzymes in typha under As stress.Exogenous P increased the activity of antioxidant enzymes of typha under As stress in sediment,but the stimulating effect was limited.Typha had more growth advantages than other ferns and bryophytes.Therefore,typha had great potential for repairing double-risk wetlands with phosphorus eutrophication and arsenic pollution.

关 键 词：外源磷 砷胁迫 抗氧化酶系统 挺水植物 

分 类 号：S661.[农业科学—果树学]