牛病毒性腹泻病毒Npro蛋白的原核表达及裂解效率  

Prokaryotic expression and splitting decomposition rate of Npro protein of bovine viral diarrhea virus

作　　者：常秋燕 郭富城 冶昡青 李凌浩 王悦萦 刘萍[1,2] 苏强 马鹏 李林杰[1,2] 金丽[1,2] 马晓霞 冯玉萍[1,2] CHANG Qiu-yan;GUO Fu-cheng;YE Xuan-qing;LI Ling-hao;WANG Yue-ying;LIU Ping;SU Qiang;MA Peng;LI Lin-jie;JIN Li;MA Xiao-xia;FENG Yu-ping(Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生物医学研究中心,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《江苏农业学报》2019年第5期1161-1166,共6页Jiangsu Journal of Agricultural Sciences

基　　金：2017年度甘肃省高等学科科研项目(2017B-80);兰州民海生物工程有限公司项目(XBMu-2015-Bc-11);西北民族大学研究生科研创新项目(Yxm2016138、Yxm2018138)

摘　　要：为了研究牛病毒性腹泻病毒N pro蛋白的催化切割效率,首先扩增GSTZ毒株的N pro基因,克隆至原核表达载体pET-28a(+)上,使其在表达菌E.coli BL21(DE 3)中表达,确定正确的N pro基因碱基序列,然后构建含有切割位点的pET-28a-N pro-GFP重组融合质粒,在原核表达系统中研究Npro蛋白的切割效率。结果显示,GSTZ毒株的N pro蛋白成功地在原核表达系统中表达,重组融合蛋白质N pro-GFP在原核表达系统中的切割效率约为60.28%。In order to research the catalytic cleavage efficiency of bovine viral diarrhea virus N pro protein,the N pro gene in GSTZ strain was amplified and cloned into prokaryotic expression vector pET-28a(+)in E.coli BL21(DE 3)to determine the correct base sequence of N pro gene.Then the pET-28a-N pro-GFP recombinant fusion plasmid containing the authentic cleavage site was constructed.The cleavage efficiency of N pro protein was studied in prokaryotic expression system.The results showed that N pro protein of GSTZ strain was successfully expressed in prokaryotic expression system,and the cleavage efficiency of recombinant fusion protein N pro-GFP in prokaryotic expression system was about 60.28%.

关 键 词：牛病毒性腹泻病毒 N^pro蛋白质 原核表达 裂解效率 

分 类 号：S852.653[农业科学—基础兽医学]