山楂标准汤剂的HPLC特征图谱研究  

Study of the standard decotion of Crataegi fructus based on HPLC feature chromatograms

作　　者：郝磊 孙福仁 高晗 李雪利 田方 李振江 李军山 HAO Lei;SUN Furen;GAO Han;LI Xueli;TIAN Fang;LI Zhenjiang;LI Junshan(Shineway Pharmaceutical Group Company Limited,Shijiazhuang,Hebei 051430,China;TCM Formula Granule Engineering Technology Research Center of Hebei,Shijiazhuang,Hebei 050200,China)

机构地区：[1]神威药业集团有限公司,河北石家庄051430 [2]河北省中药配方颗粒工程技术研究中心,河北石家庄050200

出　　处：《河北工业科技》2019年第6期422-428,共7页Hebei Journal of Industrial Science and Technology

基　　金：河北省科技计划项目(16272510)

摘　　要：为了进一步研究山楂配方颗粒的质量控制方法,以山楂标准汤剂为研究对象,采用高效液相色谱仪与GL science C18(4.6 mm×250 mm,5.0μm)色谱柱,以乙腈-0.4%磷酸为流动相,柱温为30℃,流速为1.0 mL/min,检测波长为254 nm,应用特征图谱技术以及中药指纹图谱相似度评价软件提取山楂标准汤剂特征图谱中的特征成分。研究表明,15批山楂标准汤剂中共确定了7个特征峰,分离度较好,且峰面积较大,易于识别,使用参照物标定了3个特征峰成分,各共有峰相对保留时间的RSD值小于2.5%。所建立的山楂标准汤剂HPLC特征图谱方法可靠、稳定,为山楂配方颗粒质量控制提供了一定的参考。In order to study the quality control method of Crataegi fructus formula granules,the standard decoction of Crata-egi fructus is taken as the research object,the GL science C18(4.6 mm×250 mm,5.0μm)chromatograms column are used.Acetonitrile-0.4% phosphoric acid is mobile phase,the column temperature is 30℃,the flow rate is 1.0 mL/min,and the detection wavelength is 254 nm.The characteristic components in the characteristic of the standard decoction of Crataegi fructus are extracted by using characteristic atlas technology and similarity evaluation software of traditional Chinese medicine fingerprint.The results show that there are 7 characteristic peaks in 15 batches of Crataegi fructus standard decoction.The separation degree is good,and the peak area is large and easy to identify.Three characteristic peaks are calibrated with reference substance.RSD value of relative retention time of each common peak is less than 2.5%.The established characteristic chromatographic method of Crataegi fructus standard decoction is reliable and stable,which provides a reference for the quality control of Crataegi fructus formula granules.

关 键 词：中药化学 山楂标准汤剂 特征图谱 绿原酸 金丝桃苷 异槲皮苷 

分 类 号：R284.1[医药卫生—中药学]