低温对大鼠血凝七项及血小板的影响  

Effects of hypothermia on blood coagulation and platelet in rats

作　　者：于艳君 辛华[2] 侯玉蓉 王倩钰 靳雪珂 兰添棋 孙国志[1] 张强[1] 邱洪斌[1] 王景涛[3] YU Yan-jun;XIN Hua;HOU Yu-rong;WANG Qian-yu;JIN Xue-ke;LAN Tian-qi;SUN Guo-zhi;ZHANG Qiang;QIU Hong-bin;WANG Jing-tao(Jiamusi University,Jiamusi 154007,China;The First Affiliated Hospital Of Jiamusi University,Jiamusi 154003,China;School of Basic Medicine,Jiamusi University,Jiamusi 154007,China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院,黑龙江佳木斯154003 [3]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2019年第5期20-22,共3页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省大学生创新创业项目,编号:10222033

摘　　要：目的:研究不同低温下Wistar大鼠血凝七项(凝血酶原时间PT,凝血酶原活性PT%,凝血酶原时间比值PTR,国际标准化比值INR,活化部分凝血活酶时间APTT,凝血酶时间TT,纤维蛋白原FBG)及血小板各项(血小板PLT,血小板分布率PDW,平均血小板体积MPV,大型血小板比例P-LCR)指标的变化。方法:随机选取40只Wistar大鼠,雌雄不拘,体重为180~270g,随机分为4组(常温组,20℃组,15℃组,10℃组),分别置于低温仪中1h后,尾静脉采血测量各项指标,并记录。结果:10℃组较20℃组TT有统计学意义(P<0.05),20℃组较常温组的FBG有统计学意义(P<0.05),15℃组较常温组FBG相比较有显著差异(P<0.01)。10℃组较其他3组PDW和MPV均有显著差异(P<0.01),10℃组较常温组和20℃组P-LCR有统计学意义(P<0.05,P<0.01)。结论:不同低温对大鼠血凝和血小板等有不同影响。Objective: To study the different low temperature Wistar rats blood clot seven [prothrombin time(PT), prothrombin activity(PT %), prothrombin time ratio(PTR), international standardization ratio(INR), activated partial clotting enzyme live time(APTT), thrombin time(TT), fibrinogen(FBG)] and the platelet[platelet(PLT), platelet distribution(PDW), mean platelet volume(MPV), large proportion of platelet(P-the LCR)] index changes.Methods: Forty Wistar rats, male and female, with body weight ranging from 180 to 270 g, were randomly divided into four groups(normal temperature group, 20 ℃ group, 15 ℃ group and 10 ℃ group). After being placed in a low-temperature apparatus for 1 hour, each index was measured by tail vein blood sampling and recorded.Results: TT difference between the 10 ℃ group and the 20 ℃ group was statistically significant(P<0.05);FBG difference between the 20 ℃ group and the normal temperature group was statistically significant(P<0.05);FBG difference between the 15 ℃ group and the normal temperature group was statistically significant(P<0.01).Comparison of PDW and MPV between the 10℃ group and the other three groups showed statistically significant differences(P<0.01), and the p-lcr between the 10℃ group and the normal temperature group and the 20 ℃ group showed statistically significant differences(P<0.05, P<0.01).Conclusion: different hypothermia has different effects on blood coagulation and platelet in rats.

关 键 词：低温 凝血七项 血小板 

分 类 号：R446.11[医药卫生—诊断学]