RP-Arg和NCG对营养限饲湖羊胎盘发育的影响  

Effects of dietary RP-Arg and NCG supplementation on development of maternal and fetal placenta in nutrient restriction Hu sheep during pregnancy

作　　者：孙玲伟[1,2] 王智博 安世钰[1] 张国敏 樊懿萱[1] 王锋[1] SUN Lingwei;WANG Zhibo;AN Shiyu;ZHANG Guomin;FAN Yixuan;WANG Feng(Jiangsu Livestock Embryo Engineering Laboratory,Nanjing Agricultural University,Nanjing 210095,China;Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)

机构地区：[1]南京农业大学江苏省家畜胚胎工程实验室,江苏南京210095 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《南京农业大学学报》2020年第1期125-133,共9页Journal of Nanjing Agricultural University

基　　金：国家肉羊产业技术体系资助项目(CARS-38);江苏现代农业产业技术体系(JATS[2019]419)

摘　　要：[目的]通过对妊娠母羊进行营养限饲,在日粮中添加饲喂过瘤胃精氨酸(RP-Arg)和N-氨甲酰谷氨酸(NCG),探讨对胎盘发育的影响。[方法]在妊娠35 d,将32只妊娠湖羊随机分为4组,每组8只:对照组[CG组,100%NRC(100%维持需要日粮营养水平)];限饲组(RG组,50%NRC);处理组1(ARG组,50%NRC日粮基础上添加20 g·d-1 RP-Arg);处理组2(NCG组,50%NRC日粮基础上添加5 g·d-1 NCG)。在妊娠110 d时,对所有母羊进行屠宰和样品采集,分析测定胎儿脐静脉血液中代谢物浓度、胎盘中血管生长相关因子以及mTOR通路中关键因子的表达。[结果]与限饲组相比,添加RP-Arg和NCG的胎儿体质量和胎儿质量/胎盘质量、胎儿脐静脉血和葡萄糖浓度,胎盘肉阜和子叶组织中Tie-2 mRNA表达量和p-p70S6K蛋白表达量显著升高(P<0.05),而胎儿脐静脉血中甘油和甘油三酯浓度显著降低(P<0.05)。与CG组相比,RG组中一氧化氮浓度、肉阜组织中VEGFR-2 mRNA表达量、子叶中p-4EBP1蛋白表达显著升高(P<0.05),而肉阜中p-4EBP1蛋白表达显著降低(P<0.05),但与ARG和NCG组对比无显著差异(P>0.05)。[结论]母体营养限饲影响胎盘发育,也影响肉阜和子叶组织中mTOR通路关键因子p70S6K和4EBP1蛋白及其磷酸化蛋白的表达水平,而母羊日粮中补充RP-Arg和NCG能通过调节子叶和肉阜中血管生长相关因子以及mTOR通路中关键因子的表达,促进胎盘的生长发育。[Objectives]This study aimed to evaluate the effects of rumen-protected arginine(RP-Arg)and N-carbamylglutamate(NCG)supplementation on development of maternal and fetal placenta in nutrient restriction Hu sheep during pregnancy.[Methods]From 35 to 110 days of pregnancy,32 Hu ewes carrying twin fetues were randomly divided into 4 groups,a control group(CG,n=8,100%NRC requirements for pregnant sheep),a underfed group(RG,n=8,fed 50%NRC requirements,which included 50%mineral-vitamin mixture),and 2 treatment groups(ARG and NCG,n=8,fed 50%NRC requirements supplemented with 20 g·d-1 RP-Arg or 5 g·d-1 NCG,which included 50%mineral-vitamin mixture).At 110 days of pregnancy,fetal umbilical venous plasma,maternal placenta(caruncle)and fetal placenta(cotyledons)were collected.The study evaluated the concentration of metabolites in umbilical venous plasma,the expression of this growth factor family and mTOR signaling pathway’s intermediates(mTOR,p70S6K,4EBP1)in caruncle and cotyledons.[Results]The results showed that the ratio of fetal body weights to placental weight decreased in RG group compared with CG group;however,the placental weights showed no differences among CG,ARG and NCG groups.The results showed that the ratio of fetal body weights to placental weight decreased in RG group compared with CG group;however,the placental weight,weight of cotyledons and caruncula showed no differences among CG,ARG and NCG groups.The mRNA expressions of VEGFA,VEGFR-1,PLGF1,Ang-1,and Ang-2 were not significant in cotyledons and caruncula of four groups.Significantly increased mRNA expression of VEGFR-2 was detected in RG group compared to CG,ARG,and NCG groups,but there was decreased Tie-2 mRNA expression in RG group.In RG group,mTOR signaling was inhibited,as determined by increased p 70S6K and 4EBP1 mRNA expressions,and increased p70S6K,4EBP1,and p-p70S6K proteins expression.[Conclusions]The data provide important insight into the maternal nutrition levels altering developmental programming of cotyledons and caruncula.Dietary RP-

关 键 词：湖羊 胎儿宫内生长迟缓(IUGR) 胎盘 精氨酸(Arg) N-氨甲酰谷氨酸(NCG) 

分 类 号：S814.1[农业科学—畜牧学]