自主设计试剂瓶插管改善血清CO2检测质量的研究  

Improving the Quality of Serum CO2 Detection through Intubation with a Self-Designed Reagent Bottle

作　　者：满冬亮[1] 程仕彤[1] 康辉[1] MAN Dongliang;CHENG Shitong;KANG Hui(Department of Laboratory Medicine,The First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院检验科

出　　处：《中国医科大学学报》2019年第12期1063-1066,1072,共5页Journal of China Medical University

基　　金：国家自然科学基金（81871723）

摘　　要：目的探讨血清二氧化碳(CO2)配套试剂盒在插入本实验室自主设计的试剂瓶插管后,检测血清CO2结果质量的改善情况。方法以罗氏配套CO2检测试剂盒作为原厂瓶,插入试剂瓶插管的原厂瓶作为改良瓶,在罗氏全自动生化分析仪同一检测模块的同一试剂仓中,分别运转0 h及4 h,检测质控品及40例患者新鲜血清CO2浓度,并对结果进行统计分析,判断在分析仪运转过程中改良瓶对检测血清CO2结果质量的改善情况。结果原厂瓶0 h与改良瓶0 h,原厂瓶0 h与改良瓶4 h以及改良瓶0 h与改良瓶4 h检测患者血清CO2浓度误差均<1/2TEa标准,临床可以接受;原厂瓶0 h与原厂瓶4 h检测患者血清CO2浓度误差>1/2TEa标准,临床不可接受。结论罗氏配套CO2检测试剂盒在其配套全自动生化分析仪运转中,空气中的CO2进入检测试剂,导致血清CO2检测结果偏高,出现临床不可接受的系统误差,而在配套试剂瓶中插入本实验室自主设计的试剂瓶插管后可降低由此引发的系统误差,使CO2的检测结果符合临床要求。Objective To investigate the improvement in the quality of serum carbon dioxide(CO2)test results using Roche serum CO2 kits after intubation with a self-designed reagent bottle.Methods The Roche CO2 test kit was designated as the original bottle,and the original bottle into which a reagent bottle intubation tube had been inserted was designated as the improved bottle.Both the bottles were installed in the same self-contained reagent warehouse of the automatic biochemical analyzer under the same analysis module.Next,CO2 concentrations of the quality control sampleand 40 fresh patient serum specimens were determined at 0 h and 4 h.The results were then analyzed statistically to assess improvements in the quality of serum CO2 test results when using the intubated reagent bottle during analyzer operation.Results The serum CO2 concentration error obtained when the results from testing serum using the original and improved bottles at 0 h were compared was less than half of the TEa,which was clinically acceptable.The serum CO2 concentration error obtained when the results from testing serum using the original bottle at 0 h and the improved bottle at 4 h were compared was less than half of the TEa and was clinically acceptable.The serum CO2 concentration error obtained when the results from testing serum using the improved bottle at 0 h and 4 h were compared was less than a half of the TEa,which was clinically acceptable.In contrast,the serum CO2 concentration error obtained when the results from testing serum using the original bottle at 0 h and 4 h were compared was greater than half of the TEa,which was not clinically acceptable.Conclusion The Roche CO2 test kit allows atmospheric CO2 to enter into the testing reagent during clinical examinations by the matched automatic biochemical analyzer.This results in a deviation of CO2 results(increased CO2)by the end of the operation and gradually produces a clinically unacceptable system error.However,with the intubation of the self-contained reagent bottle with the self-desi

关 键 词：试剂瓶插管 二氧化碳 偏差 

分 类 号：R446.11[医药卫生—诊断学]