PDGFR/PI3K/Akt信号通路在PDGF-BB促进体外小鼠脑星形胶质细胞增殖中的作用  

The Role of the PDGFR/PI3K/Akt Signaling Pathway in Promoting the Proliferation of Mouse Brain Astrocytes in vitro by PDGF-BB

作　　者：裴丹 李洪鹏 PEI Dan;LI Hongpeng(Department of Human Anatomy,College of Basic Medical Sciences,Jinzhou Medical University,Jinzhou 121000,China;Department of Human Anatomy,College of Basic Medical Science,China Medical University,Shenyang 110122,China)

机构地区：[1]锦州医科大学基础医学院人体解剖学教研室,辽宁锦州121000 [2]中国医科大学基础医学院人体解剖学教研室,沈阳110122

出　　处：《中国医科大学学报》2019年第12期1076-1081,共6页Journal of China Medical University

基　　金：辽宁省教育厅科学技术研究项目（JYTQN201716）

摘　　要：目的探讨血小板衍生生长因子受体(PDGFR)信号通路对血小板衍生生长因子BB(PDGF-BB)诱导的体外小鼠脑星形胶质细胞增殖的影响。方法取出生1~3 d的小鼠乳鼠大脑皮质培养星形胶质细胞,采用免疫荧光染色检测胶质纤维酸性蛋白(GFAP)的表达,对培养的星形胶质细胞的纯度进行鉴定。通过向培养细胞体系加入细胞因子PDGF-BB刺激细胞,应用PDGFR通路抑制剂AG1296进行干预,观察细胞增殖情况。Western blotting检测培养细胞系中p-PDGFR-α、p-PI3K、p-Akt及GFAP表达的变化。结果免疫荧光染色结果显示,星形胶质细胞的纯度可以达到90%。加入PDGF-BB后星形胶质细胞增殖明显增多,给予抑制剂AG1296预处理增殖受到抑制。Western blotting结果显示,加入PDGF-BB后p-PDGFRα、p-PI3K、p-Akt及GFAP表达增多(P<0.01),给予抑制剂预处理表达减少(P<0.05)。结论PDGFR信号通路参与体外小鼠脑星形胶质细胞的增殖。Objective To investigate the effects of the platelet-derived growth factor receptor(PDGFR)signaling pathway on the proliferation of mouse brain astrocytes induced by platelet-derived growth factor-BB(PDGF-BB).Methods Astrocytes were isolated from the cerebral cortex of mice from day 1 to day 3 after birth.The purity of cultured astrocytes was identified by immunofluorescence staining of glia fibrillary acidic protein(GFAP).Cultured cells were stimulated with PDGF-BB and treated with a PDGFR inhibitor,AG1296.Cell proliferation was then observed.The expression of p-PDGFR-α,p-PI3Kt,p-Akt,and GFAP was detected by Western blotting.Results The purification and identification of astrocytes showed that astrocyte cell purity reached 90%.Astrocyte proliferation increased significantly with the addition of PDGF-BB and decreased upon pre-treatment with AG1296.Further,Western blotting showed that the expression of p-PDGFR-α,p-PI3Kt,p-Akt,and p-GFAP increased after the addition of PDGF-BB(P<0.05)and decreased after pre-treatment with AG1296(P<0.05).Conclusion The PDGFR signaling pathway promotes the proliferation of mouse brain astrocytes in vitro.

关 键 词：星形胶质细胞 血小板衍生生长因子BB 血小板衍生生长因子受体 细胞增殖 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]