不同程度肾积水家兔模型在不同压力肾盂灌注下的肾损伤  

Nephron Injuries under Different Levels of Perfusion Pressure in Rabbit Models with Different Degrees of Hydronephrosis

作　　者：黄倩[1] 柳懿鹏[1] HUANG Qian;LIU Yipeng(Department of Urology,The No 1.Hospital of Wuhan,Wuhan 430022,China)

机构地区：[1]武汉市第一医院泌尿外科

出　　处：《中国医科大学学报》2019年第12期1085-1090,共6页Journal of China Medical University

基　　金：湖北省卫生计生科研基金（WJ2015Z064）

摘　　要：目的观察不同程度肾积水的家兔在不同压力肾盂灌注下的肾损伤情况。方法将54只家兔随机分为对照组(n=6)和实验组(n=48)。实验组进行手术处理,形成轻度(M组,n=24)和重度(S组,n=24)肾盂积水,M组和S组均细分为4个亚组,即M0~M3组和S0~S3组(每个亚组n=6),分别进行0、20、60、100 mmHg肾盂灌注。对照组进行麻醉和手术,但不造成肾积水。对比观察各组家兔肾组织中性粒细胞明胶酶相关载脂蛋白(NGAL)表达、超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平、线粒体膜电位(MMP)水平、线粒体空泡化比例和肾小管细胞凋亡情况。结果NGAL水平、MDA水平、线粒体空泡化比例和肾小管细胞凋亡指数,对照组最低,M0、M1、M2组与对照组相比升高(P<0.05),M3组较对照组及M0、M1、M2组均明显升高(P<0.05);S0、S1组较对照组明显升高(P<0.05),S2、S3组较对照组及S0、S1组明显升高(P<0.05)。SOD及MMP水平则相反。结论严重肾积水的肾组织在肾盂高压灌注时更容易受到损伤。Objective To compare the nephron injuries under different levels of perfusion pressure in rabbits with different degrees of hydronephrosis.Methods A total of 54 rabbits were randomly divided into control(n=6)and experimental(n=48)groups.Mild(M group,n=24)or severe(S group,n=24)hydronephrosis was induced in the experimental group by a surgical procedure.The rabbits in M and S groups were randomly divided into four subgroups(M0,M1,M2,and M3 groups;and S0,S1,S2,and S3 groups)and were perfused with 0,20,60,and 100 mmHg pressurized fluid,respectively.The renal apoptotic indices,percentages of vacuolar mitochondria,mitochondrial membrane potential(MMP),and expression levels of neutrophil gelatinase-associated lipocalin(NGAL),superoxide dismutase(SOD),and malondialdehyde(MDA)were assessed.Results The control group showed the lowest apoptotic index,percentage of vacuolar mitochondria,and NGAL and MDA expression levels in the kidney.These values were higher in M0,M1,and M2 subgroups than in the control group(P<0.05).Significant differences were observed between M3,S0,S1,S2,and S3 subgroups and their controls;between M3 and other three M subgroups;and between S0 and S1 subgroups and S2 and S3 subgroups(P<0.05).The SOD expression levels and MMPs showed the opposite trend.Conclusion Kidneys with severe hydronephrosis are more likely to be injured under high-pressure perfusion.

关 键 词：肾积水 肾盂高压灌注 肾损伤 

分 类 号：R692.2[医药卫生—泌尿科学]