黑骨藤总黄酮的提取纯化及体外抗黄嘌呤氧化酶的活性筛选  

Purification of Periploca forrestii total flavonoids and assay for the in vitro inhibitory activity on xanthine oxidase

作　　者：燕晓婷 陈华国 周欣 高赛 YAN Xiao-ting;CHEN Hua-guo;ZHOU Xin;GAO Sai(Guizhou Provincial Laboratory of Drug Quality Control and Evaluation Technology,Guizhou Normal University,Guiyang 550001,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China)

机构地区：[1]贵州师范大学贵州省药物质量控制及评价技术工程实验室,贵阳550001 [2]贵州中医药大学,贵阳550002

出　　处：《国际药学研究杂志》2019年第7期527-531,共5页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81360641);贵州省高层次创新人才培养项目(黔科合人才(2015)4033号);贵州师范大学培育项目(黔科合平台人才〔2018〕5769-27);贵州省普通高等学院科技拔尖人才支持计划项目(黔教合KY[2017]065);贵州省重大科技项目(黔科合J重大字2015-2002-3-3)

摘　　要：目的对黑骨藤总黄酮进行提取纯化,并测试总黄酮、2个黄酮类化合物槲皮素-3-O-α-L-吡喃阿拉伯糖苷(QP)和槲皮素-7-O-β-D-吡喃葡萄糖苷(QG)的体外抑制黄嘌呤氧化酶(XOD)活性,为其药效物质研究提供依据。方法使用大孔树脂联合聚酰胺法纯化黑骨藤总黄酮,根据Lambert-Beer定律测定总黄酮含量,采用紫外分光光度法测定不同浓度总黄酮及2个黄酮类化合物的体外XOD抑制活性。结果分离纯化得到了含量>95%的总黄酮。总黄酮及2个黄酮类化合物均显示有体外XOD抑制活性,且随浓度增加,抑制活性逐步增强。总黄酮的IC50为608.9μg/ml,而2个黄酮类化合物QG和QP的IC50分别为261.2和221.2μg/ml。结论黑骨藤总黄酮及2个黄酮类化合物QG和QP均显示出一定的体外XOD抑制活性。Objective To extract and purify total flavonoids from Periploca forrestii Schltr.(P.forrestii),and test the in vitro inhibitory activity of the total flavonoids and two flvaonoidal compounds in P.forrestii,so as to provide a reference for studies on the related medicinal substances in P.forrestii.Methods Total flavonoids were extracted from P.forrestii and then purified by the column chromatography on macroporous resin and polyamide columns.The content of total flavonoids was determined according to the Lambert-Beer′s law.The in vitro xanthine oxidase(XOD)inhibitory ac tivity was assayed for total flavonoids and the two flavonoidal compounds by the ultraviolet spectrophotometry.Results The purified total flavonoids had a content of more than 95%.The total flavonoids and two flavonoidal compounds all showed an inhibitory effect on XOD in vitro,with the inhibitory rate enhanced with increasing concentration.The IC50 of the total flavonoids as well as the two flavonoidal compounds,quercetin-3-O-α-L-pyranoside(QP)and quercetin-7-O-β-D-glucopyranoside(QG)were 608.9,221.2 and 261.2μg/ml,respectively.Conclusion The total flavonoids as well as the two flvaonoidal compounds QP and QG in P.forrestii all showed the in vitro inhibitory activity on XOD.

关 键 词：黑骨藤 黄嘌呤氧化酶抑制活性 总黄酮 大孔树脂联合聚酰胺 槲皮素-3-O-α-L-吡喃阿拉伯糖苷 槲皮素-7-O-β-D-吡喃葡萄糖苷 

分 类 号：R932[医药卫生—生药学]