蜡梅花被片维管束变黑的细胞结构及生理生化研究  

Study on cell structure,physiology,and biochemistry of vascular bundle blackening in petals of Chimonanthus praecox(L.) Link

作　　者：袁蒲英[1] 宋兴荣[1] 邵祎璠 Yuan Pu-Ying;Song Xing-Rong;Shao Yi-Fan(Key Laboratory of Horticultural Crops,Biology and Germplasm Enhancement in Southwest Regions,Ministry of Agriculture,Horticultural Research Institute of Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China)

机构地区：[1]四川省农业科学院园艺研究所,农业部西南地区园艺作物生物学与种质创制重点实验室,成都610066 [2]西南大学园艺园林学院,重庆400715

出　　处：《植物科学学报》2019年第6期781-787,共7页Plant Science Journal

基　　金：四川省农作物育种攻关项目(2016YZGG-11);四川省财政创新能力提升工程项目(2016ZYPZ-025);四川省财政创新能力提升工程项目(2017QNJJ-007);四川省财政专项资金项目(2016CYTS-006)~~

摘　　要：对蜡梅(Chimonanthus praecox(L.)Link)花被片维管束变黑的细胞显微结构和超微结构变化进行观察,测定和研究其生理生化指标。结果显示,中度变黑的花被片即出现维管束细胞液泡边界模糊,颗粒状物质增加,质体凝结堵塞等现象,随着维管束功能逐渐丧失,基本组织细胞失水,但表皮细胞相对饱满,以维持花朵基本功能。随着维管束变黑程度加剧,过氧化物酶(POD)活性及游离氨基酸含量持续升高,可溶性糖含量持续降低,符合花朵衰老生理特点,但丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及可溶性蛋白质含量未显著下降,表明蜡梅花朵自身对逆境的适应力较强,膜脂过氧化程度不高,大部分细胞的膜结构及内部结构受损不大。本实验可为此现象形成机理的深入研究并最终解决此问题提供一定的理论依据。We observed the microstructure and ultramicrostructure and measured the physiological and biochemical indices of vascular bundle blackening in petals of Chimonanthus praecox(L.)Link.Results showed that moderately blackened petals exhibited vacuole wall blurring,granular substance increase,and plastid coagulation and blockage.As vascular bundle function was gradually lost,the basic tissue cells became dehydrated,but the epidermal cells remained relatively full to maintain basic flower functions.As the degree of vascular bundle blackening intensified,peroxidase(POD)activity and free amino acid content increased,whereas the soluble sugar content decreased,consistent with the physiological characteristics of flower senescence.However,malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,and soluble protein content did not decrease significantly,indicating that the C.praecox flower possessed a strong ability to adapt to adversity,and the degree of membrane lipid peroxidation was not high.In addition,the membrane and internal structures of most cells were relatively undamaged.This study provides a theoretical basis for further study on the formation mechanism of this phenomenon and the final solution to this problem.

关 键 词：蜡梅 维管束变黑 细胞结构 生理生化 

分 类 号：S685.99[农业科学—观赏园艺]