IL21纳米微球抗原诱导小鼠巨噬细胞免疫应答效果的研究  

EFFECT OF NANOPARTICLE MEDIATED IL21 ANTIGEN ON IMMUNE RESPONSE IN MICE

作　　者：刘琳琳 任崇阳 程欣 姚远 张纪云 LIU Lin-lin;REN Chong-yang;CHENG xin;YAO yuan;ZHANG Ji-yun(Shandong Medical College,Linyi 276000,China)

机构地区：[1]山东医学高等专科学校

出　　处：《山东医学高等专科学校学报》2019年第6期401-403,共3页Journal of Shandong Medical College

基　　金：临沂市科技计划发展项目(No.201818065)

摘　　要：目的 探讨肿瘤疫苗的制备方法。方法 利用快速膜乳化技术与热固化法相结合制备壳聚糖季铵盐凝胶微球,分别设空白对照组、阳性对照组及共同作用组,计算各组吞噬细胞增殖率、一氧化氮释放量及巨噬细胞caspase-3的表达量。结果细胞增值率:阳性对照组为(88.86±2.31)%,共同作用组为(49.50±6.34)%。两组比较有统计学意义(t=10.10,P <0.01)。一氧化氮释放量:空白对照组、阳性对照组及共同作用组依次为(8.67±4.26)、(27.67±3.76)、(15.33±2.33)μmol/l,三组比较有统计学意义(F=22.18,P=0.002)。caspase-3表达量:阳性对照组为(1.121±0.058),共同作用组为(0.717±0.074),两组比较有统计学意义(t=7.44,P=0.002)。结论 壳聚糖季铵盐凝胶微球能有效保护核酸,显著提高吞噬细胞的活性。Objective To discuss the preparation of tumor vaccine.Methods The chitosan quaternary ammonium salt gel microspheres were prepared by the combination of rapid membrane emulsification technology and thermal curing method.The blank control group,positive control group and co-action group were set up to calculate the phagocytic cell proliferation rate,the nitric oxide release amount of each group,and the expression of caspase-3 in macrophages.Results Cell proliferation rate was(88.86±2.31)%in the positive control group and(49.50±6.34)%in the co-action group.The comparison between the two groups was statistically significant(t=10.10,P<0.01).Nitric oxide release in the blank control group,the positive control group,and the co-action group were(8.67±4.26),(27.67±3.76),and(15.33±2.33)μmol/l correspondingly,and the three groups were statistically significant(F=22.18,P=0.002).The expression of caspase-3 in the positive control group was(1.121±0.058)and(0.717±0.074)in the co-action group.The two groups were statistically significant(t=7.44,P=0.002).Conclusion Chitosan quaternary ammonium salt gel microspheres can effectively protect nucleic acids and significantly increase the activity of phagocytic cells.

关 键 词：IL21 微球疫苗 吞噬细胞 

分 类 号：R392.11[医药卫生—免疫学]