壳聚糖基可注射温敏互穿水凝胶制备及表征  

Preparation and characterization of chitosan-based injectable thermosensitive interpenetrating hydrogels

作　　者：魏荣[1] 许宁侠[1,2] 黄赛朋[1] 温惠云[1] 杨华 肖湘华 李可欣 薛伟明[1] WEI Rong;XU Ning-xia;HUANG Sai-peng;WEN Hui-yun;YANG Hua;XIAO Xiang-hua;LI Ke-xin;XUE Wei-ming(School of Chemical Engineering,Northwest University,Xi′an 710069,Shaanxi Province,China;Xi′an International University,Xi′an 710077,Shaanxi Province,China;Shaanxi Institute of Ophthalmology,Xi′an 710002,Shaanxi Province,China)

机构地区：[1]西北大学化工学院,陕西西安710069 [2]西安外事学院,陕西西安710077 [3]陕西省眼科研究所,陕西西安710002

出　　处：《化学工程》2019年第12期6-11,共6页Chemical Engineering(China)

基　　金：陕西省自然科学基础研究计划项目(2018JM2037);教育部重点实验室开放基金(ZSK2018008)

摘　　要：以β-甘油磷酸钠(GP)为羟丙基壳聚糖(HPCS)或壳聚糖季铵盐(HTCC)的温敏交联剂、氯化钙(CaCl2)为海藻酸钠(SA)的螯合交联剂,采用共混工艺,制备以壳聚糖衍生物-GP为第1网络、海藻酸钙为第2网络的可注射温敏互穿水凝胶。利用高斯软件基于氢键理论探讨凝胶温敏相变机理。利用红外光谱仪、扫描电子显微镜、热重分析仪和流变分析仪对凝胶性能进行了表征。当ρHPCS=18.75 mg/mL,ρGP=75 mg/mL,ρSA=18.33 mg/mL和ρ(CaCl2)=1.67 mg/mL时,凝胶在37℃温敏凝胶化时间为(85±15)s,压缩弹性模量为(54±15)kPa,损耗模量G″和储能模量G′比值为0.10±0.025。由此可知,HPCS-GP/Ca-Alg复合凝胶可在生理温度环境凝胶化且时间可控,凝胶机械强度良好,在组织工程领域中具有应用潜力。With hydroxypropyl chitosan(HPCS),chitosan quaternary ammonium salt(HTCC)and sodium alginate(SA)as matrix materials as well asβ-glycerol phosphate(GP)and calcium chloride(CaCl2)as crosslinkers,chitosan-based injectable thermo-sensitive interpenetrating network hydrogels were prepared.The first network was composed by CS-derivative and GP,while the second one was constituted by calcium alginate.The gelation mechanism relating hydrogen-bond was discussed by Gaussian software.The characteristics of prepared hydrogels were investigated by infrared spectroscopy,scanning electron microscopy,thermal stability analysis,compressive elastic modulus and rheological properties analysis.Corresponding toρ(HPCS)=18.75 mg/mL,ρGP=75 mg/mL,ρSA=18.33 mg/mL andρ(CaCl2)=1.67 mg/mL,the gelation time was(85±15)s at 37℃,the compressive elastic modulus was(54±15)kPa,and the ratio of loss modulus G″and storage modulus G′was 0.10±0.025.Within the samples prepared,HPCS-GP/Ca-Alg demonstrates controllable injectability and thermosensitive gelation in the range of physiological temperature as well as satisfied strength abilities,indicating its potentiality for tissue engineering application.

关 键 词：壳聚糖衍生物 海藻酸盐 互穿网络 水凝胶 温度响应 

分 类 号：TQ021.4[化学工程] O636.1[理学—高分子化学]