FTY720在成骨-破骨环境下抑制聚乙烯磨损颗粒诱导的溶骨效应  

FTY720 Inhibits Bone Destruction Induced by Polyethylene Wear Particles during the Osteoclast-osteoblast Couplin

作　　者：李平 肖楸钶 冉强 代震宇[1] 张健[2] Li Ping;Xiao Qiuke;Ran Qiang(Department of Orthopedics,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China)

机构地区：[1]重庆市中医院骨科,重庆400021 [2]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《华中科技大学学报（医学版）》2019年第6期671-675,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：重庆市卫生计生委医学科研资助项目(No.2017ZDXM006)

摘　　要：目的观察FTY720在成骨-破骨环境下对聚乙烯磨损颗粒所致溶骨效应的影响。方法构建小鼠植骨气囊模型,随机分为4组:对照组(气囊内注入生理盐水和DMSO)、FTY720组(气囊内注入生理盐水和FTY720)、聚乙烯颗粒组(气囊内注入聚乙烯颗粒和DMSO)、聚乙烯颗粒+FTY720组(气囊内注入聚乙烯颗粒和FTY720)。3周后处死小鼠取组织标本,苏木精-伊红染色对比观察各组组织标本中细胞数量,ELISA法检测各组组织标本中IL-6、TNF-α浓度,TRAP染色对比观察各组组织标本中破骨细胞数量,电镜扫描观察各组组织标本中骨片情况,RT-PCR检测各组组织标本中核转录因子NF-κB受体(RANK)mRNA表达水平。结果①颗粒组和颗粒+FTY720组气囊中细胞聚集数量高于对照组和FTY720组(均P<0.05)。②颗粒组和颗粒+FTY720组中IL-6、TNF-α浓度高于对照组和FTY720组(均P<0.05)。③颗粒组和颗粒+FTY720组中TRAP染色阳性区域面积大于对照组和FTY720组(均P<0.05),FTY720组小于对照组,颗粒+FTY720组小于颗粒组(均P<0.05)。④颗粒组和颗粒+FTY720组中骨片吸收面积大于对照组和FTY720组(均P<0.05),FTY720组小于对照组,颗粒+FTY720组小于颗粒组(均P<0.05)。⑤RANK mRNA的表达量在FTY720组和颗粒+FTY720组,分别低于对照组和颗粒组(均P<0.05)。结论 FTY720可以通过降低RANK mRNA的表达来抑制破骨细胞的分化成熟。Objective To investigate the effect of FTY720 during the osteoclast-osteoblast coupling.Methods The air pouches model was established,and the mice were divided into four groups:control group(normal saline and DMSO were introduced into the air pouches),FTY720 group(normal saline and FTY720 were introduced into the air pouches),UHMWPE group(UHMWPE and DMSO were introduced into the air pouches),UHMWPE and FTY720 group(UHMWPE and FTY720 were introduced into the air pouches).Three weeks later,all the mice are sacrificed,pouch walls and implanted calvaria were collected.The number of cells of the pouch walls were detected through HE staining;some of inflammatory cytokines,IL-6 and TNF-α in tissue homogenate were detected by ELISA,and the differentiation and maturation of osteoclast were detected through tartrate-resistant acid phosphatase staining,the scanning electron microscopy was used to examine the resorption of the bone slices.RT-PCR was used to detect the receptor activator of nuclear factor-κB(RANK)mRNA expression. Results ①The cells of the pouch walls were signficantly more in the UHMWPE group and the UHMWPE+FTY720 group than in the control group and the FTY720 group(P<0.05).②The quantity of inflammatory cytokines IL-6 and TNF-α in tissue homogenate in the UHMWPE group and the UHMWPE+FTY720 group was higher than that in the control group and the FTY720 group(P<0.05).③The TRAP staining positive area was significantly bigger in the UHMWPE group and the UHMWPE+FTY720 group than in the control group and FTY720 group,smaller in the FTY720 group than in the control group,and smaller in the UHMWPE+FTY720 group than in the UHMWPE group(P<0.05).④The area of resorption of the slices was bigger in the UHMWPE group and the UHMWPE+FTY720 group than in the control group and FTY720 group(P<0.05),smaller in the FTY720 group than in the control group,and smaller in the UHMWPE+FTY720 group than in the UHMWPE group(P<0.05).⑤The expression of RANK mRNA was lower in the FTY720 group than in the control group,and lower

关 键 词：聚乙烯颗粒 植骨气囊 鞘氨醇-1-磷酸盐 FTY720 破骨细胞 

分 类 号：R687.4[医药卫生—骨科学]