利用隐性性状及其分子标记快速选择大白菜育种纯系  

Using Recessive Traits and Their Molecular Markers to Accelerate Breeding Process of Pure Lines in Chinese Cabbage

作　　者：张烨 贺立龙 魏清岗 王红霞 李景娟 王凤德 徐少君 荆世新 孙令强 李洪雷 邓永林 高建伟 Zhang Ye;He Lilong;Wei Qinggang;Wang Hongxia;Li Jingjuan;Wang Fengde;Xu Shaojun;Jing Shixin;Sun Lingqiang;Li Honglei;Deng Yonglin;Gao Jianwei(Institute of Vegetables and Flowers,Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center,Jinan 250100,China;Institute of Science and Technology Information,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Qingdao Hefeng Seeds Co.,Ltd.,Jiaozhou 266300,China;Qingdao Jiaozhou Chinese Cabbage Research Institute,Jiaozhou 266300,China;Qingdao Agriculture and Rural Affairs Bureau,Qingdao 266071,China;Xiyou Seed Branch of Shandong Denghai Seeds Co.,Ltd./Shandong Chinese Cabbage Engineering Technology Research Center,Laizhou 261400,China)

机构地区：[1]山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100 [2]山东省农业科学院科技信息研究所,山东济南250100 [3]青岛和丰种业有限公司,山东胶州266300 [4]青岛市胶州大白菜研究所,山东胶州266300 [5]青岛市农业农村局,山东青岛266071 [6]山东登海种业股份有限公司西由种子分公司/山东省大白菜工程技术研究中心,山东莱州261400

出　　处：《山东农业科学》2019年第12期7-14,共8页Shandong Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0101801);山东省重点研发计划项目(2018GNC110003);山东省农业科学院农业科技创新工程项目“地理标识性名产蔬菜标准生产关键技术”(CXGC2016B06);国家大宗蔬菜现代农业产业技术体系济南试验站建设项目;山东省现代农业产业技术体系蔬菜创新团队建设项目(SDAIT-02-022-04)

摘　　要：加快纯系选育速度、缩短育种进程,是大白菜新品种选育的重要目标。本试验利用大白菜桔红色、无表皮毛、亮绿秆三个隐性性状及其分子标记辅助大白菜优良纯系选择,开展加快大白菜育种效率的研究。结果表明:该分子标记属于基因本身外显子区段的InDel或SNP标记,选择效率高(100%),可使大白菜优良纯系培育时间缩短3年,与常规育种相结合形成的育种技术体系,可实现桔红心、无表皮毛、亮绿秆优质大白菜新品种选育的精准分子设计。本研究结果直接来源于大白菜育种实践,具有重要的理论和实践价值。Improving the speed of breeding pure lines and shortening the breeding process were the goals of breeding new Chinese cabbage varieties.Here,we used three recessive traits,including orange color,non-trichome and bright green stem,and their molecular markers to assist the selection of fine pure lines in Chinese cabbage in order to accelerate the breeding efficiency.The results showed that the molecular markers with higher selection efficiency as 100% belonged to InDel or SNP markers of the gene exon segment,which could shorten breeding time of excellent pure lines in Chinese cabbage by three years.They could realize precise molecular designation about orange color,non-trichome and bright green stem in Chinese cabbage compared with conventional breeding.The results were directly derived from the breeding practice of Chinese cabbage,so they had important theoretical significance and practice value.

关 键 词：大白菜 隐性性状 分子标记 分离纯化 育种效率 

分 类 号：S634.103.7[农业科学—蔬菜学]