高效液相色谱法同时测定骨折挫伤胶囊中羟基红花黄色素A和大黄素含量  

Content Determination of Hydroxysafflor Yellow A and Emodin in Guzhecuoshang Capsule by HPLC

作　　者：胡小祥 何艳 HU Xiaoxiang;HE Yan(Chenzhou Institute Center for Food and Drug Control,Chenzhou,Hunan,China 423000;School of Pharmacy,Xiangnan University,Chenzhou,Hunan,China 423000)

机构地区：[1]郴州市食品药品检验检测中心,湖南郴州423000 [2]湘南学院药学院,湖南郴州423000

出　　处：《中国药业》2020年第1期54-56,共3页China Pharmaceuticals

基　　金：湖南省高等学校“双一流”药学应用特色学科[湘教通〔2018〕469号]

摘　　要：目的建立同时测定骨折挫伤胶囊中羟基红花黄色素A和大黄素含量的高效液相色谱(HPLC)法。方法色谱柱为Waters sunfire-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇(A)-0.2%磷酸溶液(B)梯度洗脱(0~9 min时15%A,9~10 min时15%A^85%A,10~25 min时85%A),检测波长为403 nm(0~10 min)、254 nm(10~25 min),流速为1.0 m L/min,柱温为35℃,进样量为10μL。结果羟基红花黄色素A、大黄素的质量浓度分别在2.12~29.68μg/m L(r=0.9998)和2.04~28.36μg/m L(r=0.9998)范围内与峰面积线性关系良好;平均加样回收率分别为99.27%和99.55%,RSD分别为1.83%和1.18%(n=9)。结论该方法简单、专属性强、重复性好,可用于骨折挫伤胶囊中红花药材和大黄药材的质量控制。Objective To establish an HPLC method for simultaneous determination of hydroxysafflor yellow A and emodin in Guzhecuoshang Capsule. Methods The chromatographic column was Waters sunfire-C18 column( 4. 6 mm × 250 mm,5 μm),with the mobile phase consisted of acetonitrile( A)-0. 2% phosphoric acid( B) with gradient elution( 0-9 min,15% A;9-10 min,15% A-85% A;10-25 min,85% A) at a flow rate of 1. 0 m L/min. The detection wavelengths were 403 nm( 0-10 min) and 254 nm( 10-25 min).The column temperature was set at 35 ℃,and the injection volume was 10 μL. Results Good linearity of hydroxysafflor yellow A and emodin was obtained in the range of 2. 12-29. 68 μg/m L( r = 0. 999 8),2. 04-28. 36 μg/m L( r = 0. 999 8),respectively. The average recovery rate were 99. 27 %( RSD = 1. 83 %,n = 9) for hydroxysafflor yellow A,99. 55 %( RSD = 1. 18 %,n = 9) for emodin.Conclusion The method is simple,feasible and repeatable,and can be used for the quality control of hydroxysafflor yellow A and emodin in Guzhecuoshang Capsule.

关 键 词：骨折挫伤胶囊 羟基红花黄色素A 大黄素 高效液相色谱法 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]