智能微流控检测芯片的构建及其Pb2+检测性能  

Fabrication of smart microfluidic chip and its Pb2+-detection performance

作　　者：汪伟[1,2] 彭减 林硕 谢锐 巨晓洁[1,2] 刘壮 褚良银[1,2] WANG Wei;PENG Jian;LIN Shuo;XIE Rui;JU Xiaojie;LIU Zhuang;CHU Liangyin(School of Chemical Engineering,Sichuan University,Chengdu 610065,Sichuan,China;State Key Laboratory of Polymer Materials Engineering,Sichuan University,Chengdu 610065,Sichuan,China)

机构地区：[1]四川大学化学工程学院,四川成都610065 [2]四川大学高分子材料工程国家重点实验室,四川成都610065

出　　处：《化工进展》2020年第1期42-48,共7页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金面上项目(21576167)

摘　　要：通过将具有Pb2+响应性的聚(N-异丙基丙烯酰胺-共聚-苯并-18冠-6丙烯酰胺)智能微凝胶与H型微通道相结合,构建了一种能便捷、灵敏、可视化检测水溶液中Pb2+浓度的新型智能Pb2+检测微流控芯片。该微流控检测芯片主要由软光刻技术构建,并结合紫外光照聚合在H型微通道中原位构建智能微凝胶。基于该微凝胶的Pb2+响应性体积相变和H型微通道中的流体流动,该微流控检测芯片能将Pb2+浓度信号有效转换为易于检测读取的、可视化的H型微通道中指示液覆盖的指示柱数目的变化信号。通过光学显微镜便捷观察测量指示液覆盖的指示柱数目的变化,实现了对水溶液中痕量Pb2+浓度的超灵敏定量检测。该微流控检测芯片为水环境中的痕量Pb2+浓度的便捷、灵敏、可视化检测提供了新策略。This work reported on fabrication of a novel smart microfluidic chip based on integration of Pb2+-responsive poly(N-isopropylacrylamide-co-benzo-18-crown-6-acrylamide) microgel and Hshaped microchannel, for facile, sensitive and visual detection of Pb2+in water. The microfluidic chip was constructed using soft lithography technique combined with in situ synthesis of the smart microgel via UVpolymerization within the H-shaped microchannel. Based on the Pb2+-responsive volume change of the smart microgel and the flow in the H-shaped microchannel, the microfluidic chip enabled efficient conversion of Pb2+concentration changes into easily detectable and readable, visual signal of number changes of indicating pillars within the H-shaped microchannel. By using a microscope for observing and measuring the number change of the indicating pillars, sensitive and quantitative detection of Pb2+concentration in water was achieved. The proposed microfluidic chip provided a new strategy for facile,sensitive and visual detection trace Pb2+in water.

关 键 词：微流体学 微通道 凝胶 体积相变 Pb2+检测信号 

分 类 号：O648.17[理学—物理化学]