半微量-微量技术在菠菜色素提取与分离中的应用  

Application of Semimicroscale and Microscale Technique in the Extraction and Separation of Spinachrome

作　　者：李志伟 康绍英 李江胜 张玲 LI Zhi-Wei;KANG Shao-Ying;LI Jiang-Sheng;ZHANG Ling(School of Chemistry and Food Engineering,Changsha University of Science Technology,Changsha 410114,China;Hunan Institute of Food Quality Supervision Inspection and Research,Changsha 410111,China)

机构地区：[1]长沙理工大学化学与食品工程学院,长沙410114 [2]湖南省食品质M监督检验研究院,长沙410111

出　　处：《化学教育（中英文）》2019年第24期55-57,共3页Chinese Journal of Chemical Education

基　　金：湖南省普通高等学校教学改革研究项目（湘教通（2015)291号）;湖南省学位与研究生教育教学改革研究课题（JG2018B081）;长沙理工大学教学改革研究项目（JG1757）

摘　　要：菠菜色素的提取与分离是高等院校化学与化工等专业开设的有机化学实验中一个非常重要的实验。该实验存在实验时间长、使用溶剂多和对环境污染严重等问题。该文采用半微量-微量技术,对菠菜叶用量、提取溶剂用量、固定相种类、层析柱大小、洗脱溶剂种类及用量等进行研究,发现以5 g菠菜叶为原料,5 mL甲醇和5 mL石油醚-甲醇(3∶2)为提取剂,4 g层析硅胶为固定相,51 mL石油醚-乙酸乙酯(3∶2)为洗脱剂在29 min内就能将菠菜中的主要色素分离开来。The extraction and separation of spinachrome constitutes a very important section in organic chemistry experiment of chemistry and chemical engineering specialty etc.But there exist some problems such as taking long experimental time,using too much solvent and making serious environmental pollution.In this paper,a series of factors including the spinach dosage,extraction solvent amount,stationary phases,column chromatography size,elution solvents and their dosage were explored using the semimicroscale and microscale technique.It has been found that the main pigments in spinach were well separated in 29 min over 4 grams of silica gel as the stationary phase using 51 mL of petroleum ether and ethyl acetate(3∶2)as the elute,after sequential extraction with 5 mL of methanol,and 5 mL of the mixture of petroleum ether and methanol(3∶2),respectively,in a scale of 5 grams of spinach leaves as the staring material.

关 键 词：菠菜色素 半微量-微量 提取 分离 

分 类 号：TS2[轻工技术与工程—食品科学与工程]