淡水双壳类背角无齿蚌AwHSP70基因克隆及多溴联苯醚-47对其表达的影响  

Molecular Characterization of AwHSP70 from Freshwater Bivalve Anodonta woodiana: Effects of PBDE-47 on Its Expression

作　　者：薛士鹏[1] 王涵 宋国英 华春秀[1] 于瑞雪 刘庆春[1] 王中晓[1] 张庆远[1] 刘丽 李冰洁 夏西超[1,2] Xue Shipeng;Wang Han;Song Guoying;Hua Chunxiu;Yu Ruixue;Liu Qingchun;Wang Zhongxiao;Zhang Qingyuan;Liu Li;Li Bingjie;Xia Xichao(College of Basic Medicine,Nanyang Medical University,Nanyang 473061,China;College of Medicine,Pingdingshan University,Pingdingshan 476000,China)

机构地区：[1]南阳医学高等专科学校基础医学部,南阳473061 [2]平顶山学院医学院,平顶山476000

出　　处：《生态毒理学报》2019年第5期168-176,共9页Asian Journal of Ecotoxicology

基　　金：河南省高等学校重点科研项目(18A330004);河南省联合基金项目(182300410123);中国博士后基金项目(2016M590143)

摘　　要：热休克蛋白(HSP)参与蛋白质折叠、细胞膜转位和错误折叠蛋白质降解等过程,提高动物对环境的应激能力和适应能力。前期研究表明,多溴联苯醚-47(PBDE-47)对背角无齿蚌具有显著的氧化应激和急性毒性效应,为探讨PBDE-47慢性毒性效应;将背角无齿蚌随机分为对照组和PBDE-47处理组,处理组用3.36μg·L-1浓度的PBDE-47进行处理,对照组用相同体积的二甲亚砜进行处理;克隆出AwHSP70基因,分析PBDE-47对AwHSP70表达的影响。结果显示,AwHSP70具有HSP70家族的标签序列,广泛分布于斧足、鳃、肝胰脏、闭壳肌、心脏、血淋巴和外套膜。PBDE-47处理可导致肝胰脏、鳃和血细胞中AwHSP70 mRNA水平显著升高。与对照组相比,PBDE-47处理组肝胰脏中AwHSP70 mRNA水平在1~15 d内增加2.79倍(P<0.01)以上;鳃中AwHSP70 mRNA水平增加3.06倍(P<0.01)以上;血淋巴中AwHSP70表达增加1.81倍(P<0.05)以上。背角无齿蚌上调AwHSP70表达有助于增强动物对PBDE-47的耐受能力.Heat shock proteins(HSPs)play an important role in adaption to environmental stress by protein folding,membrane translocation,degradation of misfolded proteins and other regulatory processes.Our previous study showed oxidative stress generated from polybrominated diphenyl ether-47(PBDE-47)could cause an acute toxicity to freshwater bivalve Anodonta woodiana,but the effect of chronic exposure need to be elucidated.In order to further investigate the chronic effect of PBDE-47,clams A.woodiana were randomly divided into the PBDE-47 treated group administrated with PBDE-47 at a concentration of 3.36μg·L^-1 and control group treated with a similar volume dimethyl sulfoxide.One complete HSP sequence was isolated from A.woodiana and named AwHSP70.AwHSP70 was widely distributed in foot,gill,hepatopancreas,adductor muscle,heart,hemocytes and mantle.Administration of PBDE-47 could result in a significant up-regulation of AwHSP70 expression in the hepatopancreas,gill and hemocytes.In the hepatopancreas,mRNA level of AwHSP70 increased more than 2.79 times(P<0.01)compared with that of control group.In the gill,during the experiment process,significant up-regulation of AwHSP70 expression showed a reversed U shape.In the hemocytes,AwHSP70 expression of PBDE-47 treated group increased more than 1.81 times(P<0.05)compared with that of control group.These results indicated that up-regulation of AwHSP70 expression accounts for enhancing adaption of bivalve A.woodiana exposed to PBDE-47.

关 键 词：PBDE-47 背角无齿蚌 AwHSP70 

分 类 号：Q459[生物学—生理学]