苯并[a]芘和9,10-二甲基蒽对皱纹盘鲍(Haliotis dis-cus hannai)早期发育的毒性研究  

Toxicity of Benzo [a] pyrene and 9,10-Dimethylanthracene on Early Development of Haliotis discus hannai

作　　者：董雪纯 魏海峰 刘长发[1,2] Dong Xuechun;Wei Haifeng;Liu Changfa(Key Laboratory of Nearshore Marine Environmental Research,Dalian 116023,China;Dalian Ocean University,Dalian 116023,China)

机构地区：[1]辽宁省近岸海洋环境科学与技术重点实验室,大连116023 [2]大连海洋大学,大连116023

出　　处：《生态毒理学报》2019年第5期212-218,共7页Asian Journal of Ecotoxicology

基　　金：海洋公益性行业科研专项(201305002,201305043);国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放课题(201309,201809);国家海洋局近岸海域生态环境重点实验室经费(201013);辽宁省教育厅服务地方项目(2017);大连海洋大学农业农村部北方海水增养殖重点实验室基金项目(2018-KF-21);国家重点研发计划项目“辽东湾污染防治与生态环境修复关键技术研究”(2019YFC1407700)

摘　　要：为探究海洋中多环芳烃(PAHs)对海洋生物的毒性作用,以皱纹盘鲍(Haliotis discus hannai)为受试生物,采用半静水式实验方式,探讨了2种多环芳烃苯并[a]芘和9,10-二甲基蒽对皱纹盘鲍早期发育的毒性效应。在不同浓度的苯并[a]芘和9,10-二甲基蒽作用下,观察皱纹盘鲍的卵子受精率、胚胎发育时间和幼体死亡率。结果表明,高浓度的多环芳烃处理组与对照组相比,卵子的受精率显著降低,胚胎发育时间和幼体死亡率显著增加,与处理浓度之间存在显著的剂量-效应关系(P<0.05)。3~12 d中,苯并[a]芘对皱纹盘鲍幼体的LC 10分别为11.6、8.18、7.67和7.66 mg·L^-1,9,10-二甲基蒽对皱纹盘鲍幼体的LC 10分别为14.91、14.11、12.82和9.64 mg·L^-1。苯并[a]芘毒性大于9,10-二甲基蒽。In order to study the ecotoxicological impact of marine polycyclic aromatic hydrocarbons(PAHs)pollutants on Haliotis discus hannai,the toxic effects of benzo[a]pyrene and 9,10-dimethylanthracene on the early development of Haliotis discus hannai were investigated under semi-static conditions.The effects of benzo[a]pyrene and 9,10-dimethylanthracene on the ovum fertilization rate,embryo development time and infant mortality were observed in different concentrations.The results showed that,compared with the blank control group,the fertilization rate in the PAHs treatment groups with different concentrations were significantly reduced,and the embryonic development time and infant mortality were significantly increased,showing a significant dose-effect relationship with the treatment concentration(P<0.05).During the 3~12 d treatment,the LC 10 of benzo[a]pyrene were 11.6,8.18,7.67 and 7.66 mg·L^-1,while that of 9,10-dimethylanthracene were 14.91,14.11,12.82 and 9.64 mg·L^-1.The toxicity of benzo[a]pyrene was higher than that of 9,10-dimethyl anthracene.

关 键 词：苯并[A]芘 9 10-二甲基蒽 皱纹盘鲍 毒性作用 LC 10 

分 类 号：X522[环境科学与工程—环境工程]