Influence of 10-(6-plastoquinonyl) decyltriphenylphosphonium on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia  

作　　者：Aleksander A Agarkov Tatyana N Popova Yana G Boltysheva 

机构地区：[1]Department of Medical Biochemistry and Microbiology, Voronezh State University

出　　处：《World Journal of Diabetes》2019年第12期546-559,共14页世界糖尿病杂志（英文版）（电子版）

摘　　要：BACKGROUND It is known that under conditions of tissue tolerance to insulin,observed during type 2 diabetes mellitus(DM2),there is an increased production of reactive oxygen species.Moreover,the free radicals can initiate lipid peroxidation(LPO)in lipoprotein particles.The concentration of LPO products can influence the state of insulin receptors,repressing their hormone connection activity,which is expressed as a reduction of the glucose consumption by cells.It is possible that reduction in glucose concentration during administration of 10-(6-plastoquinonyl)decyltriphenylphosphonium(SkQ1)to rats with DM2 may be related to the antioxidant properties of this substance.AIM To establish the influence of SkQ1 on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia.METHODS To induce hyperglycemia,rats were fed a high-fat diet for 1 mo and then administered two intra-abdominal injections of streptozotocin with a 7-d interval at a 30 mg/kg of animal weight dose with citrate buffer equal to pH 4.4.SkQ1 solution was administered intraperitoneally at a 1250 nmol/kg dose per day.Tissue samples were taken from control animals,animals with experimental hyperglycemia,rats with streptozotocin-induced glycemia that were administered SkQ1 solution,animals housed under standard vivarium conditions that were administered SkQ1,rats that were administered intraperitoneally citrate buffer equal to pH 4.4 once a week during 2 wk after 1-mo high-fat diet,and animals that were administered intraperitoneally with appropriate amount of solution without SkQ1(98%ethanol diluted eight times with normal saline solution).To determine the intensity of free radical oxidation and total antioxidant activity,we used the biochemiluminescence method.Aconitate hydratase(AH),superoxide dismutase,and catalase activities were estimated using the Hitachi U-1900 spectrophotometer supplied with software.The amount of citrate was determined by means of the Natelson method.Real-time polymerase chain reaction wBACKGROUND It is known that under conditions of tissue tolerance to insulin, observed during type 2 diabetes mellitus(DM2), there is an increased production of reactive oxygen species. Moreover, the free radicals can initiate lipid peroxidation(LPO)in lipoprotein particles. The concentration of LPO products can influence the state of insulin receptors, repressing their hormone connection activity, which is expressed as a reduction of the glucose consumption by cells. It is possible that reduction in glucose concentration during administration of 10-(6-plastoquinonyl) decyltriphenylphosphonium(Sk Q1) to rats with DM2 may be related to the antioxidant properties of this substance.AIM To establish the influence of Sk Q1 on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia.METHODS To induce hyperglycemia, rats were fed a high-fat diet for 1 mo and then administered two intra-abdominal injections of streptozotocin with a 7-d interval at a 30 mg/kg of animal weight dose with citrate buffer equal to p H 4.4. Sk Q1 solution was administered intraperitoneally at a 1250 nmol/kg dose per day.Tissue samples were taken from control animals, animals with experimental hyperglycemia, rats with streptozotocin-induced glycemia that were administered Sk Q1 solution, animals housed under standard vivarium conditions that were administered Sk Q1, rats that were administered intraperitoneally citrate buffer equal to p H 4.4 once a week during 2 wk after 1-mo high-fat diet,and animals that were administered intraperitoneally with appropriate amount of solution without Sk Q1(98% ethanol diluted eight times with normal saline solution). To determine the intensity of free radical oxidation and totalantioxidant activity, we used the biochemiluminescence method. Aconitate hydratase(AH), superoxide dismutase, and catalase activities were estimated using the Hitachi U-1900 spectrophotometer supplied with software. The amount of citrate was determined by means of the Natelson method. Real-t

关 键 词：Diabetes mellitus Free radical oxidation ANTIOXIDANTS 10-(6-plastoquinonyl)decyltriphenylphosphonium 

分 类 号：R28[医药卫生—中药学]