全基因组水平上基因家族分布及进化分析方法研究  

Research on gene family distribution and evolutionary analysis method at genome-wide level

作　　者：刘宪 于继高 肖志刚[1] 黄晟 LIU Xian;YU Jigao;XIAO Zhigang;HUANG Sheng(College of Electrical and Mechanical Engineering,Hebei Agricultural University,Baoding 071001China;College of Life Science,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]河北农业大学机电工程学院,河北保定071001 [2]华北理工大学生命科学学院,河北唐山063210

出　　处：《河北农业大学学报》2019年第6期115-121,共7页Journal of Hebei Agricultural University

基　　金：保定市科技计划项目(18ZN020)

摘　　要：随着众多植物基因组测序计划的相继完成,在全基因组水平上更加高效地解析基因家族进化机制迫在眉睫,本研究提供了1种适用于植物基因组水平检测基因家族分布及进化的计算机分析方法。本研究依据物种染色体长度、基因物理位置、候选基因Ks值等数据,结合三阶贝塞尔曲线算法,利用Python语言绘制基因染色体定位图,揭示基因间相似性及进化机制。为验证方法的实用性,本研究以亚洲棉DELLA基因家族为例进行了进化分析。结果显示在亚洲棉进化过程中GA05、GA02和GA13有明显的同源Cluster被鉴定;GA05染色体上的基因簇更活跃、变化更快;GA03基因簇发生了独立进化;GA02、GA13基因出现较晚,可能来源于GA05。本方法是1种实用的基因家族分析工具,适用于全基因组水平基因家族分布及进化分析。With the completion of many plant genome sequencing projects,it is urgent to analyze the evolutionary mechanism of gene family more efficiently at the genome-wide level.This study provides a computer analysis method for genome-wide detection of gene family distribution and evolution.Based on the data of species chromosome length,gene physical location and Ks value of candidate genes,and combined with the third-order Bessel curve algorithm,the software draws gene chromosome mapping using Python language,and reveals the similarity between genes and evolutionary mechanism.In order to verify practicability of the method,the evolutionary analysis of DELLA gene family in G.aroreum was carried out.It showed that GA05,GA02 and GA13 had obvious homologous clusters identified during the evolution of G.aroreum;the GA05 gene cluster was more active and changed faster;the GA03 gene cluster evolved independently;GA02 and GA13 genes appeared late,probably from GA05.As a practical tool for gene family analysis,the method is suitable for detection of genome-wide gene family distribution and evolutionary analysis.

关 键 词：基因家族 PYTHON 三阶贝塞尔曲线 DELLA 

分 类 号：S562.035.3[农业科学—作物学]