间充质干细胞对VX2肺癌模型化疗所致肠道损害的干预研究  

Intervention study of mesenchymal stem cells on VX2 lung cancer model of intestinal injury induced by chemotherapy

作　　者：史岩 张强 沈艳霜 Shi Yan;Zhang Qiang;Shen Yanshuang(Department of Chest Surgery,The Second Affiliated Hospital of Shandong First Medical University,Taian,Shandong,271000,China)

机构地区：[1]山东第一医科大学第二附属医院胸外科

出　　处：《当代医学》2020年第2期1-3,共3页Contemporary Medicine

基　　金：山东省泰安市泰安科技计划基金资助项目(2016NS1169)

摘　　要：目的观察间充质干细胞(MSCs)对VX2肺癌模型化疗药所致肠道损害的预防及治疗作用。方法选用体质量为2.5~3.5 kg的新西兰大白兔随机分为NS组、顺铂组、顺铂+MSCs组。在CT引导下建立VX2肺癌模型,建模成功后7 d开始给药,NS组给予等量0.9%氯化钠溶液,顺铂组注射顺铂4.4 mg/kg,顺铂+MSCs组注射顺铂第2天给予MSCs(每次1×10^6个),均于耳缘静脉注射给药,7 d给药1次(为1周期),并观察实验兔的饮食及体质量,连用3个周期。3周期结束后收集实验兔的各项指标,对实验兔肠道进行病理HE染色观察及分析。结果顺铂组在第2周期治疗中饮食及体质量出现明显下降并持续至试验结束,顺铂+MSCs组在试验初期饮食及体质量出现下降,由于MSCs的作用下该组的饮食及体质量保持稳定未再出现明显下降,差异具有统计学意义(P<0.05)。结论MSCs具有减轻化疗所致肠道损害以及改善胃肠功能的作用。Objective To observe the effects of mesenchymal stem cells(MSCs)in prevention and treatment of VX2 lung cancer model of intestinal injury induced by chemotherapy drugs.Methods 2.5-3.5 kg New Zealand rabbits were randomly divided into NS group,Cisplatin group,Cisplatin+MSCs group.The establishment of the model of VX2 lung cancer under the guidance of CT model,7 days after administration,NS group received the same amount of 0.9%sodium chloride solution,Cisplatin group was injected 4.4 mg/kg of Cisplatin,Cisplatin+MSCs group injection of Cisplatin for second days MSCs(1×10^6/times),were injected into the ear vein administration,once a week(for a period),observed the diet and body weight of experimental rabbits,after 3 cycles.The indicators collected in rabbits after 3 cycles,carries on the observation and analysis of intestinal pathological HE staining in experimental rabbits.Results In the second cycles of treatment in the Cisplatin group diet and body weight decreased significantly and continued until the end of the experiment,the Cisplatin+MSCs group in the initial period of diet and weight declined,as the group of diet and body weight remained stable under the action of MSCs has not decreased significantly,the difference was statistically significant(P<0.05).Conclusion MSCs can reduce the intestinal damage induced by chemotherapy and improve the gastrointestinal function.

关 键 词：间充质干细胞 化疗 肠道损害 VX2瘤株 肺癌 

分 类 号：R73[医药卫生—肿瘤]