缺氧条件下骨髓间充质干细胞对血管内皮细胞迁移和管腔形成的影响  

Effect of bone marrow mesenchymal stem cells under hypoxia on vascular endothelial cell migration and lumen formation

作　　者：王雅芬 苏静波 王雨生 惠延年 郭长梅 Ya-Fen Wang;Jing-Bo Su;Yu-Sheng Wang;Yan-Nian Hui;Chang-Mei Guo(Department of Ophthalmology,Xijing Hospital,Eye Institute of Chinese PLA,Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China)

机构地区：[1]空军军医大学西京医院眼科全军眼科研究所

出　　处：《国际眼科杂志》2020年第1期16-20,共5页International Eye Science

基　　金：国家自然科学基金(No.81470655,81570856,81770936)~~

摘　　要：目的:探讨缺氧条件下骨髓间充质干细胞(BMSCs)对血管内皮细胞迁移和管腔形成的影响。方法:制备3种细胞的条件培养基(CM),分别为对照组(血管内皮细胞培养基,VCM)、常氧条件下BMSCs的CM组(NCM)和缺氧条件下BMSCs的CM组(HCM)。分别用以上3种CM培养人脐静脉血管内皮细胞(HUVECs)和猴脉络膜-视网膜血管内皮细胞(RF/6A)6~24h后,观察血管内皮细胞迁移和管腔形成的能力。结果:相对于对照组和常氧组的CM,缺氧组即HCM作用下的血管内皮细胞的迁移数目和成管数量(包括成管总长度和分支数)均显著增加(均P<0.05)。在RF/6A细胞,对照组VCM、常氧组NCM和缺氧组HCM作用24h后,迁移的细胞数分别为19.00±3.61、32.33±3.06、114.00±11.53个(F=153.3,P<0.001);而HUVECs的3组细胞迁移个数则分别是76.00±9.54、122.00±18.68、307.70±25.97个(F=121.5,P<0.001)。RF/6A细胞经各组CM孵育6h后,成管数目分别为12.00±3.00、37.00±4.58、51.00±3.61个(F=81.7,P<0.0001);3组HUVECs细胞管腔形成的结果类似。结论:缺氧条件下BMSCs可促进血管内皮细胞的迁移和成管能力,这种机制可能在视网膜新生血管中发挥作用。AIM:To investigate the role of bone marrow mesenchymal stem cells(BMSCs)under hypoxia on vascular endothelial cell migration and lumen formation.METHODS:Three kinds of conditioned media(CM)were prepared which including control group(vascular endothelial cells conditioned medium,VCM),normoxic BMSCs-CM group(NCM),and hypoxic BMSCs-CM group(HCM).Then the migration and lumen formation of human umbilical vein endothelial cells(HUVECs)and monkey choroid-retinal vascular endothelial cells(RF/6A)were detected after cultured with the above three kinds of CM respectively for 6-24h.RESULTS:The numbers of cells migration and the tubes formation(including the total length of the tubes and the numbers of branches)in the hypoxic group(HCM)were increased significantly compared with the control group and the normoxic group(P<0.05).The numbers of migrated RF/6A cells were 19.00±3.61,32.33±3.06,and 114.00±11.53,respectively in control group(VCM),normoxic group(NCM)and hypoxic group(HCM)after treated for 24h(F=153.3,P<0.001).And the numbers of migrated HUVECs were 76.00±9.54,122.00±18.68,and 307.70±25.97,respectively in three groups(F=121.5,P<0.001).After incubation of RF/6A cells with three different CM for 6h,the numbers of tubes formation were 12.00±3.00,37.00±4.58,and 51.00±3.61,respectively(F=81.7,P<0.0001).The results of lumen formation of HUVECs in three groups were similar with that of RF/6A.CONCLUSION:BMSCs can promote the migration and lumen formation of vascular endothelial cells under hypoxia.This mechanism may play a role in retinal neovascularization.

关 键 词：缺氧 骨髓间充质干细胞 血管内皮细胞 迁移 管腔形成 视网膜新生血管 

分 类 号：R54[医药卫生—心血管疾病]