牛大力根化学成分及其抑制破骨细胞产生活性  

Chemical constituents of the roots of Millettia speciosa and their inhibitory effects on the formation of osteoclastic cell

作　　者：刘雅兰 张毓婷 顾琼[2] 滕希峰 何琳 LIU Ya-lan;ZHANG Yu-ting;GU Qiong;TENG Xi-feng;HE Lin(School of Pharmacy,Guangdong Pharmaceutical University;School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou 510006,China;School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Guangdong Provincial Cosmetics Engineering and Technology Research Center,Zhongshan 528458,China;School of Chinese Medicine,Guangdong Pharmaceutical University,State Administration of Traditional Chinese Medicine Key Laboratory for Production and Development of Lingnan Medicinal Material,Guangzhou 510006,China)

机构地区：[1]广东药科大学药学院 [2]中山大学药学院,广州510006 [3]广东药科大学医药化工学院广东省化妆品工程技术研究中心,中山528458 [4]广东药科大学中药学院国家中医药管理局岭南药材生产与开发重点研究室,广州510006

出　　处：《天然产物研究与开发》2019年第12期2046-2050,2188,共6页Natural Product Research and Development

基　　金：国家自然科学基金(81573310);省、市重点学科建设专项(51661001)

摘　　要：为研究牛大力(Millettia speciosa Champ.)根的化学成分及其抑制破骨细胞产生活性,采用色谱技术从牛大力根部分95%乙醇提取物中分离得到11个化合物。通过核磁共振波谱、质谱以及与文献数据比较,化合物结构鉴定为millettiaosa A(1)、高丽槐素(2)、medicarpin(3)、羽扇豆醇(4)、β-谷甾醇亚油酸酯(5)、β-谷甾醇(6)、单棕榈酸甘油酯(7)、二十七烷酸甘油酯(8)、香草醛(9)、琥珀酸甲酯(10)和1-辛醇(11)。其中化合物5和10为首次从崖豆藤属中分离得到。对化合物1~11的抑制破骨细胞产生活性进行评价,发现化合物2、3、4和6具有显著的抑制破骨细胞产生活性,其IC 50分别为2.23、1.39、2.25和1.63μM。其中化合物2为首次报道具有抑制破骨细胞产生活性。To investigate the chemical constituents and their inhibitory effects on osteoclastic cell formation activity from the roots of Millettia speciosa,eleven compounds were isolated and identified from the roots of Millettia speciosa through various column chromatography.Based on the NMR and MS spectra,their structures were identified as millettiaosa A(1),maackiain(2),medicarpin(3),lupeol(4),β-sitosterollinoleate(5),β-sitosterol(6),α-monopalmitin(7),(2 S)-1-O-heptatriacontanoyl glycerol(8),vanillin(9),methyl hydrogen succinate(10),1-octanol(11).Compounds 5 and 10 were firstly isolated from the genus Millettia.All the isolated compounds 1-11 were evaluated for their inhibitory effects on osteoclastic cell formation.Among of them,compounds 2,3,4 and 6 exhibited potent inhibition with IC 50 values of 2.23,1.39,2.25 and 1.63μM,respectively.Compound 2 was firstly reported inhibitory effects on osteoclastic cell formation activity.

关 键 词：牛大力 化学成分 结构鉴定 抑制破骨细胞产生 

分 类 号：R284[医药卫生—中药学]