非小细胞肺癌患者CD8^+CD56^+NKT细胞亚群的特性研究  

Characteristics of CD8^+CD56^+NKT cells in patients with non- small cell lung cancer

作　　者：马雅莉 叶飞[2] 袁啸 毛朝明[2] 陈德玉[2] 夏圣 MA Yali;YE Fei;YUAN Xiao;MAO Chaoming;CHEN Deyu;XIA Shen(Department of Immunology,Medical College of Jiangsu University,Zhenjiang 212013,China;Tumor Treatment Center,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,China)

机构地区：[1]江苏大学医学院免疫学教研室,江苏镇江212013 [2]江苏大学附属医院肿瘤研究院,江苏镇江212000

出　　处：《东南大学学报（医学版）》2019年第6期1012-1018,共7页Journal of Southeast University(Medical Science Edition)

基　　金：江苏省社会发展项目(BE2017696);国家自然科学基金资助项目(31570879,81871234)

摘　　要：目的:评估并探讨非小细胞肺癌(NSCLC)患者外周血中CD8^+CD56^+自然杀伤性T细胞(NKT细胞)的频数、功能特性和影响因素。方法:用流式细胞术分别检测47例NSCLC患者(肺癌组)及47例健康者(对照组)外周血CD8^+CD56^+NKT细胞频数及细胞表面CD57、Ki67、NKG2D、CD69分子的表达情况,并分析CD8^+CD56^+NKT细胞频数与患者临床病理特征的相关性。结果:肺癌组CD8^+CD56^+NKT细胞频数显著低于对照组(Z NKT=2.083,P=0.037),细胞表面的CD57、NKG2D的表达率均低于对照组(Z CD57=2.522,P=0.012;Z NKG2D=2.121,P=0.034),CD69的表达率高于对照组(Z CD69=2.159,P=0.031),差异均有统计学意义,两组Ki67分子的表达率无显著性差异(Z Ki67=0.174,P=0.862)。不同性别、年龄(以60岁为界)、病理类型(鳞癌和腺癌)、TNM分期(Ⅰ~Ⅱ期、Ⅲ期、Ⅳ期)、治疗方案(初诊、化疗、靶向治疗、化疗联合靶向治疗)的NSCLC患者CD8^+CD56^+NKT细胞频数差异均无统计学意义(均P>0.05)。结论:NSCLC患者外周血CD8^+CD56^+NKT细胞频数降低,尽管处于活化状态的细胞增多,但其细胞毒性减弱,功能耗竭,此外,CD8^+CD56^+NKT细胞频数不受性别、年龄、病理类型及TNM分期、治疗方案等因素的影响。Objective:To investigate the frequency,biological characteristics and influence factors of peripheral blood CD8^+CD56^+NKT cells in patients with non-small cell lung cancer(NSCLC).Methods:Forty-seven NSCLC patients(NSCLC group)and 47 healthy people(control group)were recruited in this study.The frequency of CD8^+CD56^+NKT cells and their expression rate of CD57、Ki67、NKG2D、CD69 in two groups were analyzed by four-color flow cytometry.Then the relationship between the frequency of CD8^+CD56^+natural killer T cells(NKT cells)and the clinical characteristics of NSCLC patients were analyzed.Results:The frequency of CD8^+CD56^+NKT cells in peripheral blood of NSCLC group was significantly lower than that of the control group(Z NKT=2.083,P=0.037).The expressing rate of CD57 and NKG2D on NKT cells were significantly reduced(Z CD57=2.522,P=0.012;Z NKG2D=2.121,P=0.034),in contrast,the expression rate of CD69 was significantly higher than that of the control group(Z CD69=2.159,P=0.031),while the difference in Ki67 between the two groups was not statistically significant(Z Ki67=0.174,P=0.862).Different gender,age(≤60 years old,>60 years old),pathological type(squamous cell carcinoma,adenocarcinoma),TNM stages(stageⅠ-Ⅱ、stageⅢ、stageⅣ)and treatment schemes(first visit,chemotherapy,targeted therapy,and chemotherapy combined with targeted therapy)did not affect the frequency of CD8^+CD56^+NKT cells in NSCLC patients,the difference was not statistically significant(P>0.05).Conclusion:The frequency of peripheral blood CD8^+CD56^+NKT cells in NSCLC patients is decreased,while the proportion of the activated cells is increased,with weakened toxicity and exhaustion.The frequency CD8^+CD56^+NKT cells is not affected by gender,age,pathological type,TNM stages and treatment schemes.

关 键 词：非小细胞肺癌 CD8+CD56+自然杀伤性T细胞 肿瘤免疫 

分 类 号：R392.9[医药卫生—免疫学]