常压室温等离子体诱变选育春雷霉素高产菌株  

Mutation of Kasugamycin High Yield Strain by Using Atmospheric and Room Temperature Plasmas

作　　者：高嫚妮 潘忠成 杨玉旺 杨宏勃 陈豪 翁婧 李蒲民 Gao Manni;Pan Zhongcheng;Yang Yuwang;Yang Hongbo;Chen Hao;Weng Jing;Li Pumin(Shaanxi Microbe Biotechnology Co.,Ltd.Weinan,Shaanxi,715500,China;Key Laboratory of Super-Light Materials and Surface Technology,Ministry of Education,College of Material Science and Chemical Engineering,Harbin Engineering University,Harbin,Heilongjiang,150001,China)

机构地区：[1]陕西麦可罗生物科技有限公司,陕西渭南715500 [2]哈尔滨工程大学材料科学与化学工程学院/教育部超轻材料与表面技术重点实验室,黑龙江哈尔滨150001

出　　处：《绿色科技》2019年第24期220-221,226,共3页Journal of Green Science and Technology

基　　金：陕西省科技厅项目(编号:2018ZKC-173);陕西省科技厅项目(编号:2019-PT-15);陕西省科技厅重大项目(编号:S2018-YF-ZDNY-0199);渭南市科技局项目(编号:ZDYF-SFGG-17-ZSG);渭南市工业公关项目(编号:2019ZDYF-GYGG-31);陕西省科协企业创新争先青年人才托举计划项目(编号:2018-1-2)

摘　　要：采用常压室温等离子体对春雷霉素生产菌株小金色链霉菌(Streptomyces microaureus)203#进行诱变育种,采用不同时间等离子体照射诱变,再经过琼脂块筛选、摇瓶初筛和复筛,获得2株春雷霉素高产菌株,摇瓶效价分别为5012μg/mL和4034μg/mL,相比原始出发菌株203的效价提高了147%和98%。To obtain Kasugamycin High-yield mutants by using atmospheric and room temperature plasmas(ARTP), streptomyces microaureus 203# was irradiated at different times by ARTP. Then the mutants were inoculated and selected using agar method inculture dishes and in shake flask for the preliminary and secondary selection. Ultimately, two high-yield mutant strains were obtained, and the titer of them reached 5012 μg/mL and 4034μg/mL, respectively. Moreover, its average titer increased 147% and 98% than the original stain 203.

关 键 词：小金色链霉菌 效价 诱变选育 春雷霉素 常压室温等离子体 

分 类 号：TQ455.5[化学工程—农药化工]