鱼藤素对非小细胞肺癌细胞体内外增殖的影响  

Effect of deguelin on proliferation of non-small cell lung cancer cell in vitro and in vivo

作　　者：吕满霞 方琼彤 陈彦洁 柳传毅 吴新荣[1,2] LYU Man-xia;FANG Qiong-tong;CHEN Yan-jie;LIU Chuan-yi;WU Xin-rong(School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China;Dept of Pharmacy,General Hospital of Southern Theater Command,PLA,Guangzhou 510010,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641 [2]中国人民解放军南部战区总医院药学部,广东广州510010

出　　处：《中国药理学通报》2020年第1期26-31,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81673608);全军后勤科研计划(No CGZ14C003)

摘　　要：目的探究鱼藤素(deguelin)对非小细胞肺癌细胞A549与裸鼠移植瘤增殖的影响。方法采用CCK-8法测定鱼藤素对A549细胞增殖的抑制作用;通过赫斯特染色和AnnexinV-FITC/PI双染实验探究鱼藤素对细胞凋亡形态以及细胞凋亡率的影响;流式细胞术测定鱼藤素对A549细胞周期的影响;此外,通过裸鼠移植瘤模型的体内实验和HE染色探究鱼藤素对裸鼠移植瘤的影响。结果鱼藤素呈浓度和时间依赖性抑制A549细胞增殖;赫斯特染色和AnnexinV-FITC/PI双染实验进一步证实了鱼藤素能诱导A549细胞凋亡;鱼藤素呈浓度依赖性阻滞A549细胞周期于G 2/M期;裸鼠移植瘤模型实验和HE染色实验发现,鱼藤素对A549裸鼠移植瘤的生长有明显抑制作用(P<0.01)。结论鱼藤素对肺癌细胞A549具有显著抑制作用,为鱼藤素的抗肿瘤活性提供新的基础。Aim To investigate the effect of deguelin on the proliferation of non-small cell lung cancer cell line A549 and nude mice.Methods CCK-8 assay was used to detect the inhibition of deguelin on proliferation of SH-SY5Y cells;Hoechst stains and AnnexinV-FITC/PI double stained method were employed to observe the apoptotic morphology and apoptotic rate;flow cytometry was applied to determine the effect of deguelin on cell cycle of A549;tumor xenograft experiment and HE staining were conducted to investigate the effect of deguelin on growth of transplanted tumor in nude mice.Results Deguelin inhibited cell proliferation of A549 dose-and time-dependently;Hoechst stains and AnnexinV-FITC/PI double stained further confirmed that deguelin could induce the apoptosis of A549 cells,while deguelin blocked A549 cell cycle in G2/M phase in concentration-dependent manner.The nude mice xenograft model and HE staining experiments showed that deguelin significantly inhibited the growth of xenografts in A549 nude mice(P<0.01).Conclusions Deguelin has a significant inhibitory effect on non-small cell lung cancer cell line A549,pointing to a basis for the study of the antitumor activity of deguelin.

关 键 词：鱼藤素 非小细胞肺癌 A549 细胞增殖 裸鼠移植瘤模型 抗肿瘤 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R329[医药卫生—基础医学]