敲减microRNA-27a通过Wnt/β-catenin信号通路抑制肾细胞癌增殖和转移  

microRNA-27a knockdown inhibits proliferation and metastasis in renal cell carcinoma through Wnt/β-catenin signaling pathway

作　　者：刘扬帆[1] 蔡政[2] 魏光敏[1] 王文廉[1] 孙星[1] 盛晶[1] 刘越[1] 屈中玉[1] LIU Yang-fan;CAI Zheng;WEI Guang-min;WANG Wen-lian;SUN Xing;SHENG Jing;LIU Yue;QU Zhong-yu(No.1 DepT of Oncology,Nanyang Hospital Affiliated to Zhengzhou University-Nanyang City Center Hospital,Nanyang Henan 473009,China;Dept of Oncology,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China)

机构地区：[1]郑州大学附属南阳市中心医院肿瘤一科,河南南阳473009 [2]云南省中医医院肿瘤科,云南昆明650021

出　　处：《中国药理学通报》2020年第1期115-121,共7页Chinese Pharmacological Bulletin

基　　金：CSCO-齐鲁肿瘤研究基金2018年度资助项目(No YQ201802-048)

摘　　要：目的探究miR-27a在肾细胞癌(RCC)增殖与转移中的作用及其机制。方法RT-qPCR检测RCC癌组织、癌旁组织、RCC细胞(Caki-1、786-O和ACHN)和正常肾小管上皮细胞(HK2)中miR-27a表达。将miR-27a inhibitor转入786-O和ACHN细胞,CCK-8实验检测细胞增殖;集落形成实验检测细胞集落形成;伤口愈合实验和Transwell实验分别检测细胞迁移与侵袭;Western blot和免疫荧光检测β-catenin表达。采用LiCl(Wnt/β-catenin信号激动剂)处理已转染miR-27a inhibitor的ACHN细胞后,检测细胞增殖、侵袭与迁移。结果RCC癌组织中miR-27a表达量明显高于癌旁组织,并随分期进展而增加。与HK2细胞相比,RCC细胞中miR-27a表达均升高。转染miR-27a inhibitor后,786-O和ACHN细胞的集落形成、细胞增殖、侵袭和迁移能力均明显降低。miR-27a inhibitor降低ACHN细胞中β-catenin的表达,LiCl能促进已转染miR-27a inhibitor的ACHN细胞的增殖、迁移与侵袭能力。结论miR-27a在RCC癌组织及RCC细胞中高表达,敲减miR-27a通过Wnt/β-catenin信号通路抑制RCC细胞增殖和转移。Aim To explore the role of miR-27a on the proliferation and metastasis of renal cell carcinoma(RCC)and its mechanism.Methods The expression of miR-27a in RCC cancer tissues,para-carcinoma tissues,RCC cells(Caki-1,786-O and ACHN)and normal renal tubular epithelial cells(HK2)were detected by RT-qPCR.After miR-27a-inhibitor transfected into 786-O and ACHN cells,cell proliferation was measured by CCK-8 assay;cell colony formation was detected by colony formation assay;cell migration and invasion were detected by cell wound healing assay and Transwell assay,respectively;the pretein expression ofβ-catenin was detected by Western blot.After transfected miR-27a inhibitor into ACHN cells then treated with LiCl(Wnt/β-catenin signal agonist),cell proliferation,migration and invasion were detected.Results The expression of miR-27a in RCC cancer tissues was significantly higher than that in para-carcinoma tissues,and increased with stage progression.Compared with HK-2 cells,the expression levels of miR-27a in RCC cells were elevated.After transfection with miR-27a inhibitor,the cell colony formation,cell proliferation,invasion and migration ability of 786-O and ACHN cells were significantly reduced.MiR-27a inhibitor reduced the expression ofβ-catenin in ACHN cells.LiCl promoted the proliferation,invasion and migration ability of ACHN cells transfected with miR-27a inhibitor.Conclusions MiR-27a is highly expressed in RCC cancer tissues and RCC cells,and knockdown of miR-27a inhibits proliferation and metastasis in RCC cells through Wnt/β-catenin signaling pathway.

关 键 词：miR-27a 肾细胞癌 WNT/Β-CATENIN信号通路 增殖 细胞迁移 细胞侵袭 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R329[医药卫生—基础医学]