PGA/PLA支架材料对膝骨关节炎组织工程软骨重建的影响  

Effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis

作　　者：管乐 康宁 GUAN Le;KANG Ning(Beijing Jingcheng Skin Hospital,Chaoyang District of Beijing,100085;Plastic Surgery Hospital, Chinese Academy of Medical Sciences,Shijingshan District, Beijing 100144,China)

机构地区：[1]北京京城皮肤医院,北京100085 [2]中国医学科学院整形外科医院,北京100144

出　　处：《海南医学院学报》2019年第24期1841-1846,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(31300801)~~

摘　　要：目的探究PGA/PLA支架材料对膝骨关节炎组织工程软骨重建的影响。方法将30只日本大耳白兔平均分为3组,健康组(H组)正常日本大耳白兔,未患膝骨关节炎;膝骨关节炎组(K组)通过模型制备使正常日本大耳白兔患有膝骨关节炎;组织工程组(T组)对膝骨关节炎日本大耳白兔进行组织工程软骨重建。每组10只白兔。通过软骨组织学评分、HE染色法、免疫组化法、Westernblot法、qRT-PCR法分析H组,K组,T组之间软骨组织学评分、软骨组织病理学及形态学变化、软骨组织中Col-Ⅱ蛋白含量及Col-ⅡmRNA的含量的差异性来探究PGA/PLA支架材料对膝骨关节炎组织工程软骨重建的影响。结果按照国际组织学评分标准对软骨组织进行评分。K组软骨组织纤维化严重,关节表面不规则,腔内积液多,缺损凹陷现象严重。T组纤维化现象减轻,表面规律,腔内积液面积减少,未出现凹陷,关节表面规律。T组和K组相比较关节炎症状、软骨组织评分均有明显好转现象(P<0.05)。H组软骨细胞分布较密集,K组分布杂乱且稀疏;H组膝关节软骨层较厚,K组软骨层薄且有破损现象;H组软骨细胞位于基质内的陷窝中,有软骨囊,K组缺损明显,没有软骨囊结构(P<0.05)。T组和K组相比较膝关节处软骨层较厚,细胞分布密集,可以看见部分细胞的软骨囊(P<0.05)。H组动物膝关节基质胶原阳性细胞较多。K组细胞数最少,软骨基质破损最严重,软骨细胞与骨基质疏松。T组和K组相比较阳性软骨基质破损减轻,细胞数量明显增多(P<0.05)。在×400的视野下,每组随机取5个视野进行计数,对比结果得出K组和T组有显著性差异(P<0.05)。通过Western blot法,对H组,K组,T组软骨组织中Col-Ⅱ蛋白含量进行免疫印迹,根据灰度分析显示,H组的Col-Ⅱ蛋白含量最高,K组最低,T组和K组相比较Col-Ⅱ蛋白含量明显升高(P<0.05)。H组日本大耳白兔软骨组织中的Col-ⅡmRNA表达量Objective To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis.Methods Thirty Japanese white rabbits were divided into three groups.The first group was healthy(Group H)normal Japanese white rabbits,without knee osteoarthritis;the second group,knee osteoarthritis group(Group k)Normal Japanese white rabbits were diagnosed with knee osteoarthritis by model preparation;Group 3,tissue engineering group(GroupT)Tissue engineering cartilage reconstruction of Japanese knee white rabbits with knee osteoarthritis.Therewere10 white rabbits per group.Throughthe cartilage histological score,HE staining,immunohistochemistry,Western blot,qRT-PCR analysis of H group,k group,T group cartilage histological score,cartilage histopathology and morphological changes,cartilage tissue,the difference in Col-Ⅱprotein content and Col-ⅡmRNA content,we investigated the effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis.Results Cartilage tissue was scored according to international histological scoring criteria.The cartilage of the k group was severely fibrotic.The surface of the joint was irregular.There were many fluids in the cavity,and the defect was severe.In the T group,the fibrosis phenomenon was alleviated;the surface was regular;the area of the effusion in the cavity was reduced;no depression occurred;and the surface of the joint was regular.Arthritis symptoms and cartilage tissue scores were significantly improved in group T and group k(P<0.05).The chondrocytes in the H group were densely distributed,and the k group was disorderly and sparse.In the H group,the cartilage layer of the knee joint was thick,and the cartilage layer in the k group was thin and damaged.The chondrocytes in the H group were located in the lacuna in the stroma,and there were cartilage sacs.The k group had obvious defects and no cartilage capsule structure(all P<0.05).Compared with k group,the cartilage layer at the knee joint was thicker a

关 键 词：PGA/PLA 支架材料 膝骨关节炎 组织工程 软骨重建 

分 类 号：R318.08[医药卫生—生物医学工程] R684.3[医药卫生—基础医学]