新橙皮苷二氢查尔酮的铜配位力与抗氧化活性  

Copper(Ⅱ)-Coordinating Force and Antioxidant Activity of Neohesperidin Dihydrochalcone

作　　者：朱思明[1,2] 万文静 王桂珍 ZHU Siming;WAN Wenjing;WANG Guizhen(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;College of Life and Geographic Sciences,Kashi University,Kashi 844006,Xinjiang,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]喀什大学生命与地理科学学院,新疆喀什844006

出　　处：《华南理工大学学报（自然科学版）》2019年第12期126-132,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(U1203183);广州市科技计划项目(201903010066);新疆自然科学基金资助项目(2018D01A03);兵团科技发展专项资金资助项目(2018AB009)~~

摘　　要：类黄酮与金属配位后往往能改善其生物活性.通过摩尔比法、等摩尔溶液连续变化法研究了新橙皮苷二氢查尔酮(NHDC)铜配合物的配位比;以紫外、红外和X-射线衍射研究了NHDC配位前后的结构变化.以DPPH法、水杨酸法和FRAP法比较NHDC配位前后抗氧化活性.结果表明:NHDC与铜的配位比为2∶1,NHDC-Cu(Ⅱ)配合物稳定性受pH影响大,在pH 7.0最稳定;NHDC与铜配位后对羟基自由基清除能力明显增强,原因可能和配位改善了NHDC的水溶性、增加NHDC的非晶化程度有关;而NHDC配位后的水溶性和生物利用度增加,归因于NHDC配位后铜对NHDC的非糖配基的"集中效应"和糖部分对非糖部分的"包埋效应".但配体配位后对脂溶性自由基如DPPH·清除能力和总还原力无明显变化.Flavonoids’biological activity can often be improved by coordinating flavonoids with metal ions.The coordination ratio of neohesperidin dihydrochalcone(NHDC)to Cu2+was studied by the method of mole ratios and the method of continuous variations of equimolar solutions.The structure of NHDC before and after the coordination was studied by UV,IR and XRD analysis.DPPH method,salicylic acid method and FRAP method were used to compare the antioxidant activity of NHDC and its complex.Results indicate that the coordination ratio of NHDC to copper is 2∶1;the stability of the NHDC-Cu(Ⅱ)complex is greatly affected by pH,and the stablest state is at pH7.0.The coordination can significantly enhance the hydroxyl radical-scavenging activity of NHDC.The reason may be that the coordination with copper ions improves the water solubility and increases the amorphous degree of NHDC.And the increase of water solubility and bioavailability after the coordination may be attributed to the"concentrated effect"of copper ions on the non-sugar ligand part of NHDC and the"embedding effect"of the sugar part of NHDC on the non-sugar part after the coordination.However,NHDC or its complex with Cu2+has no significant difference for their scavenging ability on fat-soluble free radicals like DPPH·and for their total reduction force.

关 键 词：NHDC-Cu(Ⅱ)配合物 配位特性 合成 表征 抗氧化性活性 

分 类 号：O632.54[理学—高分子化学]