FTIR方法和HPLC指纹图谱鉴别不同产地荜茇  

Identification of Piper longum L. from Different Areas by FTIR and HPLC Fingerprint Methods

作　　者：张慧文 宋晓玲 石松利 张莎莎 梁越 王焕芸 ZHANG Hui-wen;SONG Xiao-ling;SHI Song-li;ZHANG Sha-sha;LIANG Yue;WANG Huan-yun(Inner Mongolia Medical University,Huhhot 010110,China;Baotao Medical College,Baotou 014040,China;Inner Mongolia People’s Hospital,Huhhot 010017,China)

机构地区：[1]内蒙古医科大学 [2]包头医学院药学院 [3]内蒙古自治区人民医院

出　　处：《光谱学与光谱分析》2020年第1期174-178,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(81760782,81641137);内蒙古自然科学基金项目(2017BS0806,2017MS0842);内蒙古医科大学科技百万工程(联合)项目(YKD2016KJBW(LH)033)资助

摘　　要：荜茇是药食同源性中药,也是蒙、藏、维医的习用药材,临床用于治疗胃痛、腹泻等,也被用作香料或调味剂,因此其质量控制非常重要。已报道采用高效液相色谱法检测胡椒碱或荜茇宁,该方法缺乏整体性。傅里叶红外光谱法和HPLC指纹图谱联合应用考察4个产地6批次荜茇,基于共有峰率和变异峰率双指标序列分析荜茇的红外光谱,并建立荜茇的HPLC指纹图谱。结果显示两种方法对荜茇的评价结果相互印证,各批次荜茇的化学成分基本相同,质量比较稳定,其中云南产荜茇(S1)与其余5批次荜茇有一定差异。红外光谱共有峰率和变异峰率与HPLC指纹图谱均能从化学成分的角度评价药材的质量,具有整体性和综合性。荜茇的红外光谱由药材中多种化学成分的红外光谱叠加产生,而指纹图谱仅显示在波长254 nm条件下有紫外吸收的化合物,因此红外光谱在分析药材品质中更具有优势。同时考察了胡椒碱和荜茇宁的含量,云南(S1)和海南(S5, S6)较安徽亳州(S2)和广西(S3, S4)低,结果与红外和HPLC指纹图谱的结果有一定的差异,提示中药材的质量评价应更注重整体性。将FTIR和HPLC指纹图谱联合应用于荜茇的质量控制研究,两种方法互为补充,操作简便,样品使用量少,可做为药材快速鉴别的新方法,为荜茇的化学成分、质量控制和开发应用提供研究基础和依据。Piper longum L. is an edible Traditional Chinese Medicine(TCM), and used as mongolian, tibetan and uyghur medicines, and listed in the Chinese Pharmacopoeia to treat disease, such as stomachache and diarrhoea, which is also used as spice and flavoring. Active Ingredients in Piper longum L. are amide alkaloids. While reported articles mostly focus on the contents of piperine or piperlonguminine by high performance liquid chromatography, and the quality control method is relatively simple and lack of holism and innovation. This paper analyzed six batches of Piper longum L. from four areas by FTIR and HPLC fingerprint. The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of the six samples, and the dual index sequence of common peak ratio and variant peak ratio were established. Entire compositions of Piper longum L. were analyzed by HPLC fingerprint, and the contents of piperine or piperlonguminine were listed. The result showed that the evaluation results of the samples by the two methods were mutual corroboration. Six batches of Piper longum L. contained similar compositions with relatively stable quality. Among them, Piper longum L. rised in Yunnan(S1) was different from the other five batches. Results showed that common peak ratio and variant peak ratio of IR could evaluate the quality of the compositions in TCM integrally and comprehensively, as well as HPLC fingerprint. while the HPLC fingerprints only showed the compounds with ultraviolet absorption at 254 nm. Therefore, IR was suitable to analyze the composition system of complex TCM. The absorption peak strength and peak shape in the IR are the results of the interaction of various functional groups and generated by the superposition of various chemical components in the infrared spectrum of Piper longum L., The HPLC fingerprint of Piper longum L. showed 17 common peaks and the similarity verified the results of IR. Contents of piperine and piperlonguminine were higher in Piper longum L. from Bozhou and Guangxi than Yunnan and

关 键 词：荜茇 指纹图谱 HPLC 红外光谱 质量评价 

分 类 号：R282.5[医药卫生—中药学]