不同葡萄糖浓度对大鼠草酸钙结石模型的影响  

Influence of Different Glucose Concentrations on Calcium Oxalate Stone Models in Rats

作　　者：张锦山 沈绍聪 李律 王奇 尤庶敏 张才军 ZHANG Jin-shan;SHEN Shao-cong;LI Lv;WANG Qi;YOU Shu-min;ZHANG Cai-jun(School of Basic Medical Sciences,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学基础医学院

出　　处：《昆明医科大学学报》2019年第12期28-33,共6页Journal of Kunming Medical University

基　　金：昆明医科大学大学生创新创业课题基金资助项目(J1301307509)

摘　　要：目的研究不同葡萄糖浓度对大鼠肾草酸钙结石的影响。方法以目前普遍使用的肾草酸钙结石造模方法为基础,设立传统的对照组和加入不同浓度葡萄糖的实验组,同时设立空白对照。A组(生理盐水),B组(1%氯化铵+1%乙二醇),C组(5%葡萄糖+1%氯化铵+1%乙二醇),D组(10%葡萄糖+1%氯化铵+1%乙二醇)E组(15%葡萄糖+1%氯化铵+1%乙二醇)。建模完成后,收集各组大鼠24 h尿液、血清,比较各组大鼠24 h尿量、尿草酸钙结石程度、血清肌酐(Cr)、血糖(Glu)、尿素氮(BUN)、左肾重量,光镜下观察肾脏HE染色结果,比较各组大鼠肾脏病理改变程度和肾小管内草酸钙结石形成情况。结果 A组肾小管完整,尿液,肾组织中均未发现有草酸钙结晶;相比于A组,3个实验组:C组、D组、E组的肾脏、尿液草酸钙沉积在光镜(400×)下均有明显的增加(P <0.05);D组、E组的草酸钙沉积在光镜(400×)下比B组有显著提高(P <0.05);B组、C组、D组、E组的血肌酐相比于A组有明显增高(P <0.05);C组、D组、E组的血肌酐之间差异有统计学意义(P <0.05);C组、E组尿素氮相比于A组、B组差异有统计学意义(P <0.05)。结论葡萄糖能诱导大鼠产生更多结石,当大鼠每日摄入的葡萄糖浓度达到5%并继续增加时,可能会加重肾小管上皮细胞损伤,生成更多的结石。Objective To study the influence of different glucose concentrations on kidney calcium oxalate stones in SD rats. Methods Based on the currently commonly used renal calcium oxalate stone modeling method,the traditional control group and experimental group with different glucose concentrations were set up,and blank control group was set up. Blank control group :group A [normal saline], traditional control group: group B [1%ammonia chloride+1%ethylene glycol], experimental group: group C [1%ammonia chloride+1%ethylene glycol +5%glucose], group D [1% ammonia chloride+1% ethylene glycol+10% glucose], group E [1% ammonia chloride+1%ethylene glycol +15%glucose].After the modeling,We collected 24 h urine,serum samples in each group of SD rats,compared the 24 h urine volume,degree of urinary Calcium oxalate stones, serum creatinine(Cr),blood glucose(Glu),urea nitrogen(BUN),and the left kidney weight in each group of SD rats. HE staining results of kidney were observed under light microscope,we compared the renal pathological changes and the formation of calcium oxalate stones in renal tubules in each group of SD rats. Results In group A,renal tubles were intact and no calcium oxalate crystals were found in urine or renal tissue. The calcium oxalate was deposited in kidney and urine under light microscope(400×) in the experimental group C,D and E were more remarkable than in group A(P <0.05);The calcium oxalate was deposited under light microsope(400×) in group D and E were more remarkable than in group B(P < 0.05);The serum creatinine(Cr) in experimentar group B,C,D and E were more obvious than in group A(P < 0.05),there were statistically significant differences in serum creatinine(Cr) between group C,D and E(P < 0.05);Compared with group A and B,blood urea nitrogen(BUN) in group C and E had statistical significance(P < 0.05). Conclusions Glucose can induce more stones in rats. When the ratsdaily glucose intake reached 5 percent, and continued to increase, It might aggravate the damage of renal tubular epithelial cell

关 键 词：肾结石 葡萄糖 草酸钙 大鼠 

分 类 号：R691.4[医药卫生—泌尿科学]