肌内注射骨折合并脑外伤大鼠血清治疗单纯骨折的放射学及组织形态学观察  

The effect of serum therapy on the radiology and histomorphology of intramuscular injection fracture combined with brain trauma in rats

作　　者：杨博元 马勇[1] 郭杨[1] 朱亚亮 黄浩 朱爱洪 方莹 YANG Boyuan;MA Yong;GUO Yang;ZHU Yaliang;HUANG Hao;ZHU Aihong;FANG Ying(Orthop Edics Department of Traditional Chinese Medicine,The First Clinical School of Nanjing University of Traditional Chinese medicine,Nanjing,Jiangsu 210023,China;Changzhou Jintan District Hospital of traditional Chinese Medicine,Changzhou,Jiangsu 213200,China)

机构地区：[1]南京中医药大学第一临床医学院中医骨伤科,江苏南京210023 [2]常州市金坛区中医医院,江苏常州213200

出　　处：《安徽医药》2020年第1期111-114,I0003,共5页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省金坛市科技计划社会发展项目（JT2013070,JT2014061)

摘　　要：目的观察对单纯股骨骨折大鼠肌内注射骨折合并脑外伤大鼠血清(“促骨折愈合血清”)后的放射学及组织形态学变化,以确定该血清是否具备有加速骨折愈合作用。方法2017年3月至2018年3月,制备骨折合并脑外伤模型SD大鼠20只经腹主动脉采血,并分期制备治疗血清;选取20只健康大鼠提取阴性对照血清,全部加标记并冷藏。应用随机数字表进行分组,每组32只,分为实验组,对照组及空白组,96只大鼠均为股骨中段骨折。肌内注射治疗血清用于实验组,肌内注射阴性对照血清用于对照组,空白组肌内注射生理盐水。并于术后第1周、2周、3周、4周各处死8只大鼠,利用放射分析比较三组骨折的愈合情况:X射线照射、双能X线骨密度测量和组织形态学观察。结果术后X线结果提示实验组骨折愈合进程较对照组、空白组明显加快。测量骨痂体积发现:术后第2周,实验组骨痂体积(30.44±2.33)mm3,对照组骨痂体积(22.68±1.95)mm3,空白组骨痂体积(21.66±2.35)mm3,对照组和空白组骨痂体积明显小于实验组(P=0.000);术后第3周,实验组骨痂体积(26.46±1.58)mm3,对照组骨痂体积(27.29±1.66)mm3,空白组骨痂体积(26.79±2.02)mm3,三组骨痂体积比较差异无统计学意义(P=0.669)mm3;术后4周,实验组骨痂体积(22.15±1.66)mm3,对照组骨痂体积(29.99±1.32)mm3,空白组骨痂体积(29.23±1.29)mm3,对照组及空白组骨痂体积明显大于实验组(P=0.000)。术后第1周,实验组骨密度(0.128±0.123)mg/cm3,对照组骨密度(0.222±0.121)mg/cm3,空白组骨密度(0.217±0.103)mg/cm3,对比差异无统计学意义(P=0.882);术后第2周,实验组骨密度(0.266±0.100)mg/cm3,对照组骨密度(0.208±0.142)mg/cm3,空白组骨密度(0.206±0.302)mg/cm3;术后第3周,实验组骨密度(0.218±0.007)mg/cm3,对照组骨密度(0.200±0.012)mg/cm3,空白组骨密度(0.189±0.069)mg/cm3;术后第4周,实验组骨密度(0.225±0.009)mg/cm3,对照组骨密度(0.190±0.100Objective To observe the radiological and histomorphological changes of rats with intramuscular injection fractures and brain injury("fracture⁃promoting serum")in rats with simple femoral fractures to determine whether the serum has accelerated frac⁃ture healing.Methods Twenty SD rats with fracture combined with traumatic brain injury were collected from the abdominal aorta and the serum was prepared in stages.20 healthy rats were randomly selected to extract the negative control serum,all labeled and refrigerated.The others were divided into treatment group,control group and blank group by random number table,with 32 in each group.96 rats were middle femur fractures.Intramuscular injection of serum was used in the treatment group,intramuscular injection of negative control serum was used for the control group,and blank group was intramuscularly injected with physiological saline.Eight rats were sacrificed at the first week,2 weeks,3 weeks,and 4 weeks after operation.The healing of the three groups was com⁃pared by radiological analysis:X⁃ray irradiation,dual⁃energy X⁃ray bone density measurement and histomorphological observation.Results The X⁃ray results showed that the healing process of the treatment group was significantly faster than that of the control group and the blank group.Measurement of osteophyte volume found:in the second week after surgery,the osteophyte volume(30.44±2.33)mm3 in the treatment group,the osteophyte volume in the control group(22.68±1.95)mm3,and the volume of the epiphysis in the blank group(21.66±2.35)mm3.The volume of the callus of the control group was significantly smaller than that of the treatment group(P=0.000).At the third week after surgery,the volume of the callus was 26.46±1.58 mm3,and the volume of the control group was(27.29±1.66)mm3,the volume of the epiphysis in the blank group(26.79±2.02)mm3,the difference in the volume of the three groups of osteophytes was not statistically significant(P=0.669)mm3;after 4 weeks,the volume of the epiphy⁃sis in t

关 键 词：股骨骨折/药物疗法 注射 肌肉内 血清 颅脑损伤 骨折愈合 骨痂 骨生成 骨密度 大鼠 Sprague⁃Dawley 

分 类 号：R68[医药卫生—骨科学]