SSR分子标记鉴定两系杂交水稻“荃两优2118”的种子纯度  

Detection of Seed Purity of Two-line Hybird Rice “Quanliangyou 2118” by SSR Marker Technique

作　　者：王立广 张翔[1] 黄贯刘 汪仁举 康圣好 熊延军[1] 张奎[1] WANG Li-guang;ZHANG Xiang;HUANG Guan-liu(Anhui Hua’an Seed Co.,Ltd.,Hefei,Anhui 230031)

机构地区：[1]安徽华安种业有限责任公司

出　　处：《安徽农业科学》2020年第1期42-43,共2页Journal of Anhui Agricultural Sciences

摘　　要：采用SSR分子标记技术鉴定两系杂交水稻“荃两优2118”的种子纯度。从48对SSR引物中筛选到2组引物(RM7120、RM8277)。随机选取“荃两优2118”种子6组,每组样品随机取192棵种子苗种进行纯度检测,结果显示同一样品SSR标记与田间鉴定结果接近,最大、最小差异分别为2.05%和0.23%。因此,SSR标记技术适用于“荃两优2118”的种子纯度鉴定。The seed purity of two-line hybird rice“Quan liangyou 2118”was identified using simple sequence repeat(SSR)molecular markers.Two pairs of primers(RM7120 and RM8277)were screened out from 48 pairs of SSR primers.A total of 2 pairs of primers were used to test the purity of 6 groups of the“Quan liangyou 2118”,with 192 seed seedings in each group.The SSR identification results were basically agreed with the field identification results,the difference of which ranged from 0.23%-2.05%.The research results indicated that SSR markers could be utilized to identify the purity of“Quan liangyou 2118”.

关 键 词：SSR分子标记 两系杂交水稻 种子纯度 田间鉴定 

分 类 号：S511[农业科学—作物学]