转TaCOMT-3D基因小麦的农艺性状评价  

Evaluation of Agronomic Traits of Transgenic Wheat with TaCOMT-3D

作　　者：周淼平[1] 张增艳[2] 姚金保[1] 王化敦[1] 杨学明[1] 张鹏[1] 余桂红[1] 马鸿翔[1] ZHOU Miao-ping;ZHANG Zeng-yan;YAO Jin-bao(Institute of Grain Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《安徽农业科学》2020年第1期102-105,共4页Journal of Anhui Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2016ZX08002001);江苏省农业科学院基金项目(6111687)

摘　　要：TaCOMT-3D基因是小麦木质素合成的关键基因,也是小麦抵御纹枯病菌侵染的重要基因。为了评估转基因小麦中TaCOMT-3D基因组成型表达对农艺性状的影响,4个遗传稳定的转基因株系及其受体对照扬麦16用于TaCOMT-3D基因检测、不同器官表达分析验证、纹枯病抗性鉴定、小区产量试验以及主要农艺性状的考察。结果显示,转基因株系均能检测到导入的TaCOMT-3D基因并且该基因能组成型表达;转基因株系与受体对照扬麦16相比,纹枯病抗性显著提高;除了部分转基因株系的主穗穗长、主穗粒数、籽粒蛋白含量与受体对照差异显著外,转基因小麦的生育期、株高、产量性状、主穗性状和籽粒性状与受体对照均没有显著差异。试验结果表明,TaCOMT-3D基因的组成型表达对转基因小麦的主要农艺性状影响不明显。TaCOMT-3D gene is a key gene in wheat lignin synthesis and is also an important gene resistant to wheat sharp eyespot.To assess the effect on agronomic traits of the transgenic wheat by TaCOMT-3D,four varieties with stable heredity and their receptor Yangmai 16 were used to detect the TaCOMT-3D,analyze the expression of the gene in different organs,evaluate the resistance to wheat sharp eyespot,test plot yield and survey the major agronomic traits.The results showed that the TaCOMT-3D gene could be detected in all transgenic lines and expressed constitutively in different organs.Compared with Yangmai 16,the resistance to wheat sharp eyespot improved significantly in all transgenic lines.There were no significant differences in wheat growth period,plant height,yield traits,main spike traits and grain traits except for significant differences in grain protein content,ear length and number of grains of main spike between some transgenic lines and receptor control.The results indicated that the constitutive expression of TaCOMT-3D had no significant effect on the main agronomic traits of transgenic wheat.

关 键 词：小麦 TaCOMT-3D 转基因 纹枯病 农艺性状 

分 类 号：S503.2[农业科学—作物学]