基于iTRAQ技术的桉树对桉树枝瘿姬小蜂抗性蛋白质组学分析  

Proteomics Analysis of Resistance of Eucalyptus to Leptocybe invasa Based on iTRAQ Technique

作　　者：王怡 冯丽贞[1] 张清华[1] 陈全助[1] 吴晖[1] WANG Yi;FENG Li-zhen;ZHANG Qing-hua(Forestry College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]福建农林大学林学院

出　　处：《安徽农业科学》2020年第1期154-156,共3页Journal of Anhui Agricultural Sciences

摘　　要：为了利用iTRAQ技术研究受桉树枝瘿姬小蜂危害后桉树叶片的差异蛋白,选择被姬小蜂危害后第24 h的桉树叶,采用iTRAQ定量蛋白质组学技术开展研究,在uniprot_Quercus中共鉴定到蛋白质1687个。按照表达倍数变化1.2倍以上(上调大于1.2倍或者下调小于0.83倍)且P<0.05的标准筛选差异表达蛋白质。其中,C_vs_T组的上调差异表达蛋白质有110个,下调差异表达蛋白质92个。经过KEGG富集分析,筛选出的差异表达蛋白质富集于苯丙烷生物合成(phenylpropanoid biosynthesis)、核糖体(ribosome)、单菌素生物合成(monobactam biosynthesis)、有机含硒化合物代谢(selenocompound metabolism)、丙酮酸盐代谢(pyruvate metabolism)、脂肪酸延长代谢(fatty acid elongation)、丙酸代谢(propanoate metabolism)7条通路。In order to explore serum differential proteins in Eucalyptus leaves after Leptocybe invasa infestation using iTRAQ technology.Eucalyptus leaves of 24 h after being harmed by Leptocybe invasa were selected,and the iTRAQ quantitative proteomics technique was used to study the subject.A total of 1687 proteins were identified in uniprot_Quercus.The differentially expressed proteins were screened according to the standard of expression fold change of 1.2 times or more(upregulation of more than 1.2 times or down-regulation of less than 0.83 times)and P<0.05.Among them,there were 110 differentially expressed proteins in the C_vs_T group and 92 differentially expressed proteins.After KEGG enrichment analysis,the differentially expressed proteins were enriched in seven pathways:Phenylpropanoid biosynthesis,Ribosome,Monobactam biosynthesis,Selenocompound metabolism,Pyruvate metabolism,Fatty acid elongation,and Propanoate metabolism.

关 键 词：桉树枝瘿姬小蜂 蛋白质组学 ITRAQ 

分 类 号：S763.43[农业科学—森林保护学]