氧化石墨烯集成腐蚀型81°倾斜光栅生物传感器  

Biosensor Based on Cladding-etched 81° Tilted Fiber Grating Coated with Graphene Oxide

作　　者：王亚杰 罗彬彬[1] 石胜辉[1] 蒋上海 吴德操 吴胜昔[2] 鲁友铭 巫涛江 赵明富[1] WANG Ya-jie;LUO Bin-bin;SHI Sheng-hui;JIANG Shang-hai;WU De-cao;WU Sheng-xi;LU You-ming;WU Tao-jiang;ZHAO Ming-fu(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing University of Technology,Chongqing 400054,China;School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China;Chongqing Energy College,Chongqing 402247,China)

机构地区：[1]重庆理工大学光纤传感与光电检测重庆市重点实验室,重庆400054 [2]重庆理工大学药物化学与分子药理学重庆市重点实验室,重庆400054 [3]重庆能源职业学院,重庆402247

出　　处：《光子学报》2019年第12期81-88,共8页Acta Photonica Sinica

基　　金：国家自然科学基金(Nos.61875026,61505017);重庆市科委基础研究与前沿探索项目(Nos.cstc2018jcyjAX0122,cstc2019jcyjmsxmX0093);重庆市教委科技项目(No.KJQN201801121);重庆理工大学研究生创新项目(No.ycx2018231)~~

摘　　要：为了改善普通81°倾斜光纤光栅在生化检测中灵敏度低、检测极限不理想等问题,提出一种基于氧化石墨烯修饰腐蚀型81°倾斜光纤光栅的牛血清蛋白生物传感器,分析了该传感器的原理与传感特性.使用氢氟酸溶液腐蚀减小光栅直径,提高其对折射率的灵敏度,并用氧化石墨烯修饰光栅,然后将牛血清蛋白单克隆抗体固定于光栅表面,用于对牛血清蛋白的特异性检测.实验结果表明,氧化石墨烯集成腐蚀型81°倾斜光纤光栅生物传感器对牛血清蛋白的检测范围为0.15~15 nmol/L,检测极限为~0.165 nmol/L,其线性响应区域的灵敏度为~182 pm/(nmol·L^-1),传感器的检测范围较氧化石墨烯集成标准直径81°倾斜光纤光栅有所降低,但其灵敏度提高了5.3倍,且检测极限有较大的改善.In order to improve the sensitivity and limit of detection of 81°tilted fiber grating,bovine serum albumin immunosensor based on graphene oxide modified cladding-etched 81°tilted fiber grating was proposed,the principle and sensing properties of the sensor were analyzed.Refractive index sensitivity of 81°tilted fiber grating was improved by etching with hydrofluoric acid.Then the etched 81°tilted fiber grating was coated with graphene oxide,and bovine serum albumin monoclonal antibody were modified on the surface of 81°tilted fiber grating for the specific detection of bovine serum albumin.Experimental results show that the detection range of the immunosensor is 0.15~15 nmol/L,limit of detection^0.165 nmol/L,and the sensitivity in its linear response region is^182 pm/(nmol·L^-1).Although the detection range decrease,the sensitivity is^5.3 times of that of the un-etched graphene oxide coated 81°tilted fiber grating,and the limit of detection is also improved.

关 键 词：光纤光学 倾斜光纤光栅 氧化石墨烯 牛血清蛋白 生物传感器 

分 类 号：TN253[电子电信—物理电子学]